Combined light microscopy and attenuated total reflection fourier transform infrared spectroscopy for integration of biofilm structure, distribution, and chemistry at solid-liquid interfaces.

Applied and Environmental Microbiology
P A SuciG G Geesey

Abstract

Reflected differential interference contrast microscopy and attenuated total reflection Fourier transform infrared spectroscopy were used to obtain complementary data on the structural and chemical properties of a biofilm. This information was obtained nondestructively, quasisimultaneously, and in real time, thereby permitting the verification of time-dependent relationships between the biofilm's population structure, distribution, and interfacial chemistry. The approach offers opportunities to examine these relationships on a variety of substrata in the presence of a bulk aqueous phase under controlled hydrodynamic conditions.

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Citations

Oct 6, 1998·Applied and Environmental Microbiology·J P BusalmenD J Schiffrin
Oct 4, 2012·Applied Spectroscopy·Rohit Bhargava
Apr 2, 1999·Journal of Endourology·G ReidJ Denstedt
Nov 3, 2017·The Review of Scientific Instruments·Vladislav LirtsmanDan Davidov
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Jan 17, 2013·Biofouling·Ahmed HouariPatrick Di Martino
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Jul 15, 2015·Applied Microbiology and Biotechnology·Ruiyong ZhangMario Vera
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Dec 8, 2015·Applied and Environmental Microbiology·M FischerT F Krauss
Jul 14, 2001·Colloids and Surfaces. B, Biointerfaces·P A. Suci, G G. Geesey

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