Combined mRNA microarray and proteomic analysis of eutopic endometrium of women with and without endometriosis

Human Reproduction
A FassbenderT M D'Hooghe

Abstract

An early semi-invasive diagnosis of endometriosis has the potential to allow early treatment and minimize disease progression but no such test is available at present. Our aim was to perform a combined mRNA microarray and proteomic analysis on the same eutopic endometrium sample obtained from patients with and without endometriosis. mRNA and protein fractions were extracted from 49 endometrial biopsies obtained from women with laparoscopically proven presence (n= 31) or absence (n= 18) of endometriosis during the early luteal (n= 27) or menstrual phase (n= 22) and analyzed using microarray and proteomic surface enhanced laser desorption ionization-time of flight mass spectrometry, respectively. Proteomic data were analyzed using a least squares-support vector machines (LS-SVM) model built on 70% (training set) and 30% of the samples (test set). mRNA analysis of eutopic endometrium did not show any differentially expressed genes in women with endometriosis when compared with controls, regardless of endometriosis stage or cycle phase. mRNA was differentially expressed (P< 0.05) in women with (925 genes) and without endometriosis (1087 genes) during the menstrual phase when compared with the early luteal phase. Proteomic analysis ...Continue Reading

References

May 1, 1980·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·T E Sensky, D T Liu
Feb 24, 2001·Neural Networks : the Official Journal of the International Neural Network Society·J A SuykensB De Moor
Feb 28, 2002·Human Reproduction Update·Thomas M D'Hooghe, Sophie Debrock
Apr 13, 2002·Annals of the New York Academy of Sciences·Daniel W Cramer, Stacey A Missmer
Jun 7, 2003·Acta Obstetricia Et Gynecologica Scandinavica·Gunhild Kalleberg HusbyMette Haase Moen
Mar 27, 2004·Molecular Human Reproduction·Yvonne AbsengerElke Winterhager
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Oct 27, 2004·Molecular Human Reproduction·Anna P PonnampalamPeter A W Rogers
Nov 16, 2004·Lancet·Linda C Giudice, Lee C Kao
Jan 25, 2005·Cellular and Molecular Life Sciences : CMLS·C PunyadeeraP Groothuis
Mar 8, 2005·Lifetime Data Analysis·Il Do Ha, Youngjo Lee
Jun 28, 2005·Human Reproduction·Stephen KennedyUNKNOWN ESHRE Special Interest Group for Endometriosis and Endometrium Guideline Development Group
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
May 9, 2006·Gynecologic and Obstetric Investigation·T M D'HoogheC Meuleman
Feb 10, 2007·Expert Review of Proteomics·Terence C W Poon
Jan 25, 2008·Human Reproduction·H DassenP Groothuis
Nov 18, 2009·Human Reproduction·Johannes L H Evers, André C Van Steirteghem
May 14, 2010·Human Reproduction Update·K E MayC M Becker
Aug 31, 2010·Fertility and Sterility·Cleophas M KyamaThomas M D'Hooghe
Nov 11, 2010·Reproductive Sciences·Lusine Aghajanova, Linda C Giudice
Dec 15, 2010·Human Reproduction Update
Jan 25, 2012·Obstetrics and Gynecology·Amelie FassbenderThomas D'Hooghe

❮ Previous
Next ❯

Citations

Oct 2, 2013·Human Reproduction Update·Signe AltmäeAndres Salumets
Jun 14, 2013·Current Opinion in Obstetrics & Gynecology·Richard O Burney
Apr 4, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·L WangW Sun
Feb 19, 2013·Fertility and Sterility·Amelie FassbenderThomas D'Hooghe
Dec 4, 2015·Mass Spectrometry Reviews·Huiliang YangYuquan Wei
Sep 20, 2015·Reproductive Biomedicine Online·Juan A Garcia-VelascoCarlos Simon
Jul 31, 2013·Proteomics. Clinical Applications·A B SivaSisinthy Shivaji
Aug 2, 2014·Scandinavian Journal of Clinical and Laboratory Investigation·Richard O Burney
Aug 5, 2015·BioMed Research International·Amelie FassbenderLinda Giudice
Jun 21, 2016·Fertility and Sterility·Lusine AghajanovaLinda C Giudice
Nov 6, 2015·Reproductive Biomedicine Online·Signe Altmäe, Lusine Aghajanova
Apr 12, 2013·Biology of Reproduction·Juliana MeolaRui Alberto Ferriani
Feb 13, 2016·Reproductive Sciences·Essam R OthmanAyman Al-Hendy
Apr 21, 2016·The Cochrane Database of Systematic Reviews·Devashana GuptaVicki Nisenblat
Nov 4, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Nilufer RahmiogluKrina T Zondervan
Dec 18, 2018·Proteomics. Clinical Applications·Simone Ferrero
Mar 29, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vanessa DuboisFrank Claessens
Jun 1, 2020·Journal of Assisted Reproduction and Genetics·Erika PrašnikarTanja Kunej
Aug 29, 2020·Biology of Reproduction·Xi GuoXiaoyan Chen
Sep 24, 2020·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Tahreem SaharSaima Wajid
Aug 29, 2018·Journal of the Endocrine Society·Dustin M BrownRosalia C M Simmen
Aug 8, 2014·Journal of Proteome Research·Anni P VehmasGarry L Corthals

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.