Combined passive and active microrheology study of protein-layer formation at an air-water interface

Langmuir : the ACS Journal of Surfaces and Colloids
Myung Han LeeRobert L Leheny

Abstract

We investigate the mechanical properties of layers of the protein beta-lactoglobulin during their formation at the air-water interface using a combination of passive and active microrheological techniques. The passive microrheology, which employs multiple particle tracking measurements using spherical colloids, indicates that the interfacial rheology evolves over time through three stages as protein adsorbs at the interface: (i) an increase in viscosity, (ii) a period of spatial heterogeneity in which the interface contains elastic and viscous regions, and (iii) the development of a uniformly rigid elastic film. Varying solution pH between pH = 5.2, the isoelectric point of beta-lactoglobulin, and pH = 7.0 has no qualitative effect on this mechanical evolution. The active microrheology, which employs ferromagnetic nanowires rotating in response to magnetic torques, similarly shows an increasing interfacial viscosity at early times and evidence of mechanical heterogeneity at intermediate times. However, at late times, the nanowire mobility becomes strongly pH dependent. For pH = 5.2, the layer responds as a rigid elastic film to the stress imposed by the wire. For pH = 7.0, it displays a viscous response that contrasts with the ...Continue Reading

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Citations

Sep 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Zhengzheng LiaoIvan J Dmochowski
Oct 14, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Marek CieplakDaniel H Reich
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Feb 16, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Yan GaoPrajnaparamita Dhar
Feb 11, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Gergana M RadulovaAlex Lips
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May 2, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Yani Zhao, Marek Cieplak
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Sep 10, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Jin-Mi JungRaffaele Mezzenga
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