Combined photodynamic and photothermal induced injury enhances damage to in vivo model blood vessels

Lasers in Surgery and Medicine
Kristen M KellyJ Stuart Nelson

Abstract

The degree of port wine stain (PWS) blanching following pulsed dye laser (PDL) therapy remains variable and unpredictable. Because of the limitations of current PDL therapy, alternative treatment approaches should be explored. The objective was to evaluate a novel methodology for selective vascular damage, combined photodynamic (PDT) and photothermal (PDL) treatment, using the in vivo chick chorioallantoic membrane (CAM) model. Thirty microliters of benzoporphyrin derivative monoacid ring A (BPD) solution was administered intraperitoneally into chick embryos at day 12 of development. Study groups were: (1) control (no BPD, no light); (2) BPD alone; (3) continuous wave irradiation (CW) alone (576 nm, 60 mW/cm2, 125 seconds); (4) CW + PDL; (5) BPD+PDL; (6) PDT (BPD+CW); (7) PDL alone (585 nm, 4 J/cm(2)); and (8) PDT+PDL (BPD + CW followed immediately by PDL). Vessels were videotaped prior to, and at 1 hour post-intervention and then assessed for damage based on the following scale: 0, no damage; 1, coagulation; 1.5, vasoconstriction; 2.0, coagulation+vasoconstriction; 2.5, angiostasis; 3.0, hemorrhage. Damage scores were weighted by vessel "order." PDT + PDL resulted in significantly (P < 0.01) more severe vascular damage than wa...Continue Reading

References

Mar 1, 1991·Journal of the American Academy of Dermatology·R Ashinoff, R G Geronemus
Nov 1, 1986·The Journal of Investigative Dermatology·J M GardenJ A Parrish
May 1, 1995·The Journal of Investigative Dermatology·U HohenleutnerM Landthaler
Nov 1, 1994·The Journal of Investigative Dermatology·S KimelM W Berns
Apr 16, 1998·The New England Journal of Medicine·C M van der HorstM J van Gemert
Oct 10, 1998·The British Journal of Dermatology·S W Lanigan
Jan 29, 2000·The Journal of Investigative Dermatology·M M TsoukasN Kollias
Feb 15, 2000·Journal of Photochemistry and Photobiology. B, Biology·M J Hammer-WilsonM W Berns
Apr 23, 2002·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Kristen M KellyJ Stuart Nelson
Apr 1, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Marie J Hammer-WilsonMichael W Berns
Aug 20, 2003·The Journal of Investigative Dermatology·Maritza A Middelkamp-HupR Rox Anderson
Mar 9, 2004·Lasers in Surgery and Medicine·Sol KimelJ Stuart Nelson

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Citations

Oct 16, 2012·Lasers in Medical Science·Clóvis GreccoCristina Kurachi
Sep 3, 2011·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Haixia QiuNaiyan Huang
Nov 6, 2008·Expert Review of Anticancer Therapy·Shlomit Halachmi, Moshe Lapidoth
May 30, 2012·Seminars in Cutaneous Medicine and Surgery·Amit M PatelKristen M Kelly
Feb 7, 2012·Journal of the American Academy of Dermatology·Jennifer K ChenMichal Heger
Nov 26, 2009·Photodiagnosis and Photodynamic Therapy·Joshua A TournasKristen M Kelly
Nov 26, 2009·Photodiagnosis and Photodynamic Therapy·Kai-Hua YuanZheng Huang
Sep 5, 2008·Photodiagnosis and Photodynamic Therapy·Kristen Kelly
Jul 31, 2007·Journal of the American Academy of Dermatology·Zaid F Jasim, Julian M Handley
Oct 19, 2005·Dermatologic Therapy·Susanne Astner, R Rox Anderson
Oct 28, 2008·Lasers in Surgery and Medicine·Jennifer ChannualKristen M Kelly
Mar 26, 2016·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Bernard ChoiJ Stuart Nelson
Jan 24, 2006·Clinics in Dermatology·Macrene Alexiades-Armenakas
Apr 13, 2005·Otolaryngologic Clinics of North America·Silvia Mioc, Mary-Ann Mycek
May 18, 2005·Lasers in Surgery and Medicine·Eric F Bernstein, Douglas B Brown
Sep 21, 2005·Archives of Facial Plastic Surgery·Kristen M KellyJ Stuart Nelson
Nov 20, 2008·Archives of Facial Plastic Surgery·Edward C Wu, Brian J F Wong
Jun 1, 2010·The Journal of Dermatological Treatment·Wei LiKenji Hashimoto
Jan 26, 2021·Photobiomodulation, Photomedicine, and Laser Surgery·Yue HanXiaoxi Lin

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