PMID: 9557873Apr 29, 1998Paper

Combined preparative enzymatic synthesis of dTDP-6-deoxy-4-keto-D-glucose from dTDP and sucrose

Glycoconjugate Journal
A SteinLothar Elling

Abstract

dTDP-6-deoxy-4-keto-D-glucose (1), the common intermediate in the biosyntheses of the manifold deoxysugars, was synthesized on a gram-scale by the combination of sucrose synthase and dTDP-D-glucose 4,6-dehydratase in a fed batch, starting the reaction with dTDP. This process allowed a dTDP conversion with a 100% rate. An easy and efficient three-step purification with anion-exchange chromatography and gel filtration gave 1.1 g of 1 in an overall yield of 73%. This work realizes a first step for an economic access to activated deoxysugars.

Citations

Oct 24, 2003·Biotechnology and Bioengineering·Jongmin OhHei Chan Lee
Jun 25, 2005·Chembiochem : a European Journal of Chemical Biology·Lothar EllingWolfgang Piepersberg
Feb 24, 2001·Nature·K M Koeller, C H Wong
May 13, 2003·The Journal of Biological Chemistry·Andreas PfoestlPaul Messner
Jan 17, 2012·Analytical Biochemistry·Ting YangMaor Bar-Peled
Mar 31, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yun-Gon KimYung-Hun Yang

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