Combined RNAi and localization for functionally dissecting long noncoding RNAs.

Nature Methods
Debojyoti ChakrabortyFrank Buchholz

Abstract

Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional characterization and localization approaches to study lncRNAs. Using this technique we identified transcripts that regulate mouse embryonic stem cell identity.

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Citations

Apr 9, 2013·Human Molecular Genetics·Debarshi MustafiKrzysztof Palczewski
Mar 13, 2014·International Journal of Hematology·Juan R Alvarez-DominguezHarvey F Lodish
Oct 5, 2013·Science China. Life Sciences·Juanjuan ZhuXiaofei Zheng
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Oct 17, 2012·EMBO Reports·Wenqian HuHarvey F Lodish
Jul 24, 2015·Cellular & Molecular Biology Letters·Dan Qin, Cunshuan Xu
Aug 16, 2014·ELife·Andrew R BassettChris P Ponting
Oct 8, 2013·Genomics, Proteomics & Bioinformatics·Wenwen JiaJiuhong Kang
Jun 11, 2014·Briefings in Functional Genomics·Youhuang BaiMing Chen
Mar 31, 2015·BioMed Research International·Yun XiaoXia Li
Sep 7, 2016·Trends in Genetics : TIG·Bethany SignalMarcel E Dinger
Jun 21, 2017·Journal of Cellular and Molecular Medicine·Saeede SalehiAbbas Behzad-Behbahani
Oct 31, 2015·The EMBO Journal·Julieta Aprea, Federico Calegari
Apr 26, 2015·Journal of Biomolecular Screening·Mirko TheisFrank Buchholz
Jun 28, 2018·SLAS Technology·John Lalith Charles Richard, Pieter Johan Adam Eichhorn
Apr 12, 2019·The Biochemical Journal·Emily J ShieldsRoberto Bonasio
Mar 18, 2021·Disease Models & Mechanisms·Jens HansenWolfgang Wurst
Jun 3, 2021·Cancers·Lidia BorkiewiczIlona Telejko

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