Combined simulation and experimental study of large deformation of red blood cells in microfluidic systems

Annals of Biomedical Engineering
David J QuinnSubra Suresh

Abstract

We investigate the biophysical characteristics of healthy human red blood cells (RBCs) traversing microfluidic channels with cross-sectional areas as small as 2.7 × 3 μm. We combine single RBC optical tweezers and flow experiments with corresponding simulations based on dissipative particle dynamics (DPD), and upon validation of the DPD model, predictive simulations and companion experiments are performed in order to quantify cell deformation and pressure-velocity relationships for different channel sizes and physiologically relevant temperatures. We discuss conditions associated with the shape transitions of RBCs along with the relative effects of membrane and cytosol viscosity, plasma environments, and geometry on flow through microfluidic systems at physiological temperatures. In particular, we identify a cross-sectional area threshold below which the RBC membrane properties begin to dominate its flow behavior at room temperature; at physiological temperatures this effect is less profound.

References

Apr 1, 1979·Biophysical Journal·R Waugh, E Evans
Jan 1, 1976·Biophysical Journal·E Evans, R M Hochmuth
May 1, 1988·Journal of Biomechanical Engineering·R S Frank, R M Hochmuth
Jan 1, 1987·Annual Review of Physiology·S Chien
Oct 1, 1970·Microvascular Research·R M HochmuthS P Sutera
Oct 1, 1970·Microvascular Research·S P SuteraR M Hochmuth
Mar 1, 1968·Circulation Research·P B Canham, A C Burton
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·N Mohandas, E Evans
Nov 1, 1996·Journal of Biomechanical Engineering·T W Secomb, R Hsu
Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi Noguchi, Gerhard Gompper
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Manouk AbkarianHoward A Stone
Mar 1, 2006·Journal of Biomechanical Engineering·Prosenjit BagchiAleksander S Popel
Mar 22, 2007·Annual Review of Biomedical Engineering·Sheldon WeinbaumEdward R Damiano
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·J P MillsSubra Suresh
Jul 31, 2007·PLoS Pathogens·Meher AntiaPradipsinh K Rathod
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·J M HigginsL Mahadevan
Sep 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·YongKeun ParkSubra Suresh
Oct 15, 2008·Physical Review Letters·Igor V Pivkin, George Em Karniadakis
Mar 10, 2009·Bulletin of Mathematical Biology·C Pozrikidis
Apr 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·J Liam McWhirterGerhard Gompper
Apr 28, 2009·Physical Review Letters·Gerrit DankerChaouqi Misbah
May 21, 2010·Biophysical Journal·Dmitry A FedosovGeorge Em Karniadakis
Jan 1, 2005·Annual Review of Fluid Mechanics·Aleksander S Popel, Paul C Johnson

Citations

May 15, 2013·Biomechanics and Modeling in Mechanobiology·Dmitry A FedosovGerhard Gompper
May 1, 2012·Annual Review of Biomedical Engineering·Keith H K WongJoe Tien
Nov 8, 2014·Biomicrofluidics·Justyna CzerwinskaDominik E Uehlinger
Jul 2, 2014·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Xuejin LiGeorge Em Karniadakis
May 9, 2012·Acta Biomaterialia·Antoine Jérusalem, Ming Dao
Feb 9, 2016·Interface Focus·Xuejin LiGeorge Em Karniadakis
Aug 20, 2016·Rheologica Acta·Alireza YazdaniGeorge Em Karniadakis
Sep 1, 2015·The European Physical Journal. E, Soft Matter·S Kumar Ranjith
Jan 2, 2015·Biomicrofluidics·Nancy F Zeng, William D Ristenpart
Apr 16, 2016·Biomechanics and Modeling in Mechanobiology·L L XiaoB M Fu
Jul 3, 2013·Journal of Biomechanics·Dong XuJohn Williams
Jun 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Igor V PivkinSubra Suresh
Nov 20, 2014·The Analyst·Balpreet Singh AhluwaliaOlav Gaute Hellesø
May 24, 2018·International Journal for Numerical Methods in Biomedical Engineering·Sazid Zamal HoqueB S V Patnaik
Aug 23, 2018·Lab on a Chip·Azeem AhmadBalpreet Singh Ahluwalia
Sep 1, 2018·Lab on a Chip·Maria Alejandra Lizarralde IragorriWassim El Nemer
Aug 1, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Chun-Chieh ChangJian Chen
Sep 19, 2017·PLoS Computational Biology·Kirill LykovIgor V Pivkin
Nov 5, 2016·Journal of Biomechanical Engineering·Xuejin LiGeorge Em Karniadakis
Sep 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yuhao QiangE Du
Oct 3, 2019·Journal of the Royal Society, Interface·B J M van RooijA G Hoekstra
Nov 28, 2016·Biomechanics and Modeling in Mechanobiology·Sara Salehyar, Qiang Zhu
Oct 17, 2017·Analytical Methods : Advancing Methods and Applications·Hesam BabahosseiniMasoud Agah
Aug 20, 2017·Annals of Biomedical Engineering·Jun Hong ParkSang Joon Lee
Oct 19, 2017·Frontiers in Physiology·Lena WiegmannVartan Kurtcuoglu
Nov 26, 2020·Microfluidics and Nanofluidics·Mehdi NikfarYaling Liu
Mar 8, 2017·Journal of the Mechanical Behavior of Biomedical Materials·Kostyantyn R PartolaGeorge Lykotrafitis
Dec 12, 2012·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Bojan Božič, Gregor Gomišček

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