Combined stool-based multiplex PCR and microscopy for enhanced pathogen detection in patients with persistent diarrhoea and asymptomatic controls from Côte d'Ivoire

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
S L BeckerLutz von Müller

Abstract

Infectious diarrhoea ranks among the leading causes of morbidity worldwide. Although most acute diarrhoeal episodes are self-limiting, the diagnosis and treatment of persistent diarrhoea (≥2 weeks) are cumbersome and require laboratory identification of the causative pathogen. Stool-based PCR assays have greatly improved the previously disappointing pathogen detection rates in high-income countries, but there is a paucity of quality data from tropical settings. We performed a case-control study to elucidate the spectrum of intestinal pathogens in patients with persistent diarrhoea and asymptomatic controls in southern Côte d'Ivoire. Stool samples from 68 patients and 68 controls were obtained and subjected to molecular multiplex testing with the Luminex(®) Gastrointestinal Pathogen Panel (GPP), microscopy and rapid antigen detection tests for the diagnosis of diarrhoeagenic pathogens. Overall, 20 different bacteria, parasites and viruses were detected by the suite of diagnostic methods employed. At least one pathogen was observed in 84% of the participants, and co-infections were observed in >50% of the participants. Enterotoxigenic Escherichia coli (32%), Giardia intestinalis (29%) and Shigella species (20%) were the predomina...Continue Reading

References

May 22, 2009·Gastroenterology·Sean W PawlowskiRichard Guerrant
Mar 12, 2011·Genome Medicine·James M KinrossJeremy K Nicholson
Feb 19, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F ChappuisC P Yansouni
Apr 30, 2013·The Lancet Infectious Diseases·Cedric P YansouniMarleen Boelaert
May 30, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C MengelleJ Izopet
Oct 18, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E WesselsE C J Claas
Nov 28, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E HalliganS Goldenberg
Mar 14, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Y ZboromyrskaJ Vila
Apr 18, 2014·The New England Journal of Medicine·Herbert L DuPont
Jul 16, 2014·The Lancet Infectious Diseases·Michel Drancourt

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Citations

May 13, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G Dubourg, F Fenollar
Jun 29, 2016·Infectious Diseases of Poverty·Shun-Xian ZhangXiao-Nong Zhou
Nov 5, 2016·PLoS Neglected Tropical Diseases·Sören L BeckerJürg Utzinger
Feb 18, 2017·Journal of Parasitology Research·Tiago MendesSilmara Marques Allegretti
Jan 5, 2018·Genes·Sandra Christine Andersen, Jeffrey Hoorfar
Nov 13, 2018·PLoS Neglected Tropical Diseases·Jackie KneeJoe Brown
May 28, 2019·Tropical Medicine and Infectious Disease·Hassan K M FofanaSören L Becker
Jun 28, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Yoav F Alkan, Michael L Alkan
Apr 22, 2017·Parasites & Vectors·Sylvia Afriyie Squire, Una Ryan
Sep 12, 2015·Transactions of the Royal Society of Tropical Medicine and Hygiene·Sören L BeckerLutz von Müller
Apr 21, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·David A ElsemoreDwight D Bowman
Jul 5, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R D'AlfonsoF Berrilli
Apr 24, 2020·Heliyon·Harriet U UgbokoJohn O Oyewale
Nov 15, 2020·Journal of Microbiological Methods·Luis Enrique Jerez PueblaLucy J Robertson
Jun 16, 2021·Journal of Gastroenterology and Hepatology·Readon TehKewin Tien Ho Siah

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