Combined strategies for optimal detection of the contact point in AFM force-indentation curves obtained on thin samples and adherent cells

Scientific Reports
Núria Gavara

Abstract

Atomic Force Microscopy (AFM) is a widely used tool to study cell mechanics. Current AFM setups perform high-throughput probing of living cells, generating large amounts of force-indentations curves that are subsequently analysed using a contact-mechanics model. Here we present several algorithms to detect the contact point in force-indentation curves, a crucial step to achieve fully-automated analysis of AFM-generated data. We quantify and rank the performance of our algorithms by analysing a thousand force-indentation curves obtained on thin soft homogeneous hydrogels, which mimic the stiffness and topographical profile of adherent cells. We take advantage of the fact that all the proposed algorithms are based on sequential search strategies, and show that a combination of them yields the most accurate and unbiased results. Finally, we also observe improved performance when force-indentation curves obtained on adherent cells are analysed using our combined strategy, as compared to the classical algorithm used in the majority of previous cell mechanics studies.

References

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Citations

Jun 15, 2017·IEEE Transactions on Nanobioscience·Mi LiYuechao Wang
Jan 22, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Minami TanakaKaoru Katoh
Nov 22, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Alexander J Steeves, Fabio Variola
Apr 16, 2020·Nanoscale·Pablo D GarciaRicardo Garcia
Dec 13, 2018·Journal of Nanobiotechnology·Xiangying DengZhaoyang Zeng
Mar 3, 2019·Scientific Reports·Luis R FloresNúria Gavara
Jan 16, 2020·International Journal of Molecular Sciences·Xiaoli ZhangNúria Gavara
Jun 27, 2020·International Journal of Molecular Sciences·Michael C Keeling, Núria Gavara
May 11, 2016·Journal of Cellular Physiology·Ignasi JorbaDaniel Navajas
Sep 28, 2016·Microscopy Research and Technique·Núria Gavara
May 27, 2020·Nature Materials·Johannes RheinlaenderKristian Franze
Aug 8, 2020·Small·Stefano ChiodiniPilar Cea
Oct 22, 2020·Journal of Nanobiotechnology·Ines LüchtefeldMassimo Vassalli
Oct 22, 2020·Communications Biology·Marina L YuberoJavier Tamayo
Jul 27, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Eros AzzaliniSerena Bonin

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Methods Mentioned

BETA
AFM
fluorescence imaging
Atomic Force Microscopy

Software Mentioned

Matlab

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