Combined sustained delivery of basic fibroblast growth factor and administration of granulocyte colony-stimulating factor: synergistic effect on angiogenesis in mouse ischemic limbs

Journal of Endovascular Therapy : an Official Journal of the International Society of Endovascular Specialists
Oju JeonByung-Soo Kim

Abstract

To investigate whether the efficacy of a single angiogenic therapy (sustained delivery of basic fibroblast growth factor [bFGF] or administration of granulocyte colony-stimulating factor [G-CSF]) can be enhanced further by combining the therapies. One day after surgical induction of hind-limb ischemia, groups of 6 mice were randomized to receive either no treatment, sustained delivery (SD) of bFGF, endothelial progenitor cell (EPC) mobilization with G-CSF administration, or a combination of bFGF SD + G-CSF administration. G-CSF administration increased significantly (p < 0.05) the number of EPC lineages (CD34 + /AC133 + cells) in both peripheral blood and bone marrow compared to no G-CSF administration. The bFGF SD and G-CSF administration individually increased the capillary and arteriole densities significantly versus no treatment (capillary density: 659 +/- 48/mm2 and 385 +/- 59/mm2, respectively, versus 280 +/- 28/mm2; p < 0.05; arteriole density: 34 +/- 9/mm2 and 41 +/- 6/mm2, respectively, versus 15 +/- 2/mm2; p<0.05). Importantly, bFGF SD + G-CSF further increased the capillary and arteriole densities compared to either strategy alone (capillary density: 786 +/- 40/mm2 versus 659 +/- 48/mm2 and 385 +/- 59/mm2, respective...Continue Reading

References

Mar 1, 1991·The Journal of Clinical Investigation·F BussolinoA Mantovani
Aug 1, 1994·The Journal of Clinical Investigation·K HaradaM Simons
Apr 1, 1994·The American Journal of Physiology·E F UngerS E Epstein
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·D OrlicP Anversa
Mar 6, 2002·Journal of Vascular Surgery·John BlebeaIan S Zagon
Dec 1, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Tiffany M PowellRichard O Cannon
Aug 10, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oju JeonByung-Soo Kim
Oct 15, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Mejeong LeeToyoaki Murohara

❮ Previous
Next ❯

Citations

May 5, 2011·International Journal of Molecular Sciences·Jiming LiLei Li
Jul 4, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oju JeonByung-Soo Kim
Aug 10, 2007·Ultrasound in Medicine & Biology·Yuichi MiyakeMasakazu Kohno
Jul 9, 2020·Tissue Engineering. Part B, Reviews·Maximilian M MengerTina Histing
Sep 14, 2017·Stem Cells International·Shaohua LiaoZhenhua Zhou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.