Combined Therapy with SS31 and Mitochondria Mitigates Myocardial Ischemia-Reperfusion Injury in Rats

International Journal of Molecular Sciences
Fan-Yen LeeHon-Kan Yip

Abstract

Myocardial ischemia-reperfusion (IR) injury contributes to adverse cardiac outcomes after myocardial ischemia, cardiac surgery, or circulatory arrest. In this study, we evaluated the ability of combined SS31-mitochondria (Mito) therapy to protect heart cells from myocardial IR injury. Adult male SD rats (n = 8/each group) were randomized: group 1 (sham-operated control), group 2 (IR, 30-min ischemia/72 h reperfusion), group 3 (IR-SS31 (2 mg intra-peritoneal injection at 30 min/24 h/48 h after IR)), group 4 (IR-mitochondria (2 mg/derived from donor liver/intra-venous administration/30 min after IR procedure)), and group 5 (IR-SS31-mitochondria). In H9C2 cells, SS31 suppressed menadione-induced oxidative-stress markers (NOX-1, NOX-2, oxidized protein) while it increased SIRT1/SIRT3 expression and ATP levels. In adult male rats 72 h after IR, left ventricular ejection fraction (LVEF) was highest in sham-operated control animals and lowest in the IR group. LVEF was also higher in IR rats treated with SS31-Mito than untreated IR rats or those treated with Mito or SS31 alone. Areas of fibrosis/collagen-deposition showed the opposite pattern. Likewise, levels of oxidative-stress markers (NOX-1, NOX-2, oxidized protein), inflammatory m...Continue Reading

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Citations

Jan 17, 2020·Journal of Applied Physiology·Gerald S SupinskiLeigh Ann P Callahan
Jul 9, 2020·Journal of Cellular and Molecular Medicine·Tien-Hung HuangFan-Yen Lee
Jun 21, 2020·Metabolites·Mateusz M Tomczyk, Vernon W Dolinsky
Aug 28, 2020·Frontiers in Physiology·Yu-Min ZhangRu-Xing Wang
Mar 16, 2021·Journal of Ethnopharmacology·Xiaofen LiWenjun Zou
May 1, 2021·International Journal of Molecular Sciences·Xiaonan SunHongyu Qiu
Jul 3, 2021·Oxidative Medicine and Cellular Longevity·Jia HuangChengbin Wang
Aug 25, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jui-Ning YehHon-Kan Yip

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