Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips.

Molecular Plant Pathology
Qiaochun WangJari P T Valkonen

Abstract

Accumulation of viruses in vegetatively propagated plants causes heavy yield losses. Therefore, supply of virus-free planting materials is pivotal to sustainable crop production. In previous studies, Raspberry bushy dwarf virus (RBDV) was difficult to eradicate from raspberry (Rubus idaeus) using the conventional means of meristem tip culture. As shown in the present study, it was probably because this pollen-transmitted virus efficiently invades leaf primordia and all meristematic tissues except the least differentiated cells of the apical dome. Subjecting plants to thermotherapy prior to meristem tip culture heavily reduced viral RNA2, RNA3 and the coat protein in the shoot tips, but no virus-free plants were obtained. Therefore, a novel method including thermotherapy followed by cryotherapy was developed for efficient virus eradication. Heat treatment caused subcellular alterations such as enlargement of vacuoles in the more developed, virus-infected cells, which were largely eliminated following subsequent cryotherapy. Using this protocol, 20-36% of the treated shoot tips survived, 30-40% regenerated and up to 35% of the regenerated plants were virus-free, as tested by ELISA and reverse transcription loop-mediated isotherma...Continue Reading

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Citations

Jan 22, 2011·Archives of Virology·N ValasevichA Kvarnheden
Dec 15, 2010·Virus Genes·Michael J MelzerJohn S Hu
Sep 23, 2011·Trends in Plant Science·Muthappa Senthil-Kumar, Kirankumar S Mysore
Feb 17, 2009·Trends in Plant Science·Qiaochun Wang, Jari P T Valkonen
Jan 13, 2018·Plant Cell Reports·Min-Rui WangQiao-Chun Wang
Feb 23, 2019·Planta·Jaime A Teixeira da SilvaJudit Dobránszki
Apr 9, 2019·Plant Disease·Lei ZhaoQiaochun Wang
Oct 17, 2018·Plant Methods·Min-Rui WangQiao-Chun Wang
Jan 1, 2021·Virusdisease·María Teresa González-ArnaoCarlos Alberto Cruz-Cruz

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