Combined transcript, proteome, and metabolite analysis of transgenic maize seeds engineered for enhanced carotenoid synthesis reveals pleotropic effects in core metabolism

Journal of Experimental Botany
Mathilde DecourcelleGerhard Sandmann

Abstract

The aim of this study was to assess whether endosperm-specific carotenoid biosynthesis influenced core metabolic processes in maize embryo and endosperm and how global seed metabolism adapted to this expanded biosynthetic capacity. Although enhancement of carotenoid biosynthesis was targeted to the endosperm of maize kernels, a concurrent up-regulation of sterol and fatty acid biosynthesis in the embryo was measured. Targeted terpenoid analysis, and non-targeted metabolomic, proteomic, and transcriptomic profiling revealed changes especially in carbohydrate metabolism in the transgenic line. In-depth analysis of the data, including changes of metabolite pools and increased enzyme and transcript concentrations, gave a first insight into the metabolic variation precipitated by the higher up-stream metabolite demand by the extended biosynthesis capacities for terpenoids and fatty acids. An integrative model is put forward to explain the metabolic regulation for the increased provision of terpenoid and fatty acid precursors, particularly glyceraldehyde 3-phosphate and pyruvate or acetyl-CoA from imported fructose and glucose. The model was supported by higher activities of fructokinase, glucose 6-phosphate isomerase, and fructose 1...Continue Reading

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Citations

Aug 16, 2016·Transgenic Research·Jürgen BreitenbachGerhard Sandmann
Mar 4, 2017·Current Opinion in Biotechnology·Giovanni Giuliano
Mar 1, 2020·International Journal of Molecular Sciences·Junqin ZhouXiaofeng Tan
Apr 3, 2020·Briefings in Functional Genomics·Upasna Chettry, Nikhil K Chrungoo
Sep 29, 2020·Frontiers in Plant Science·Sara Durán-SoriaJosé G Vallarino
Nov 15, 2016·Frontiers in Plant Science·Dipak GayenKarabi Datta
Oct 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Can BaysalPaul Christou
May 6, 2021·The Plant Genome·Lekha T PazhamalaRajeev K Varshney
Jul 31, 2021·Plant Communications·David B MedeirosAlisdair R Fernie

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Methods Mentioned

BETA
transgenic
electrophoresis
genetic modification

Software Mentioned

GeneChip Expression Console
BioSynlab
GeneChip Command Console
Affymetrix
Chemstation ( Agilent
Data Analysis
Mascot
Volcano
GeneSpring GX12
Ideal

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