Combined transcriptome and genome analysis of single micrometastatic cells

Nature Biotechnology
Christoph A KleinG Riethmüller

Abstract

In human cancer, early systemic spread of tumor cells is recognized as a leading cause of death. Adjuvant therapies are administered to patients after complete resectioning of their primary tumors to eradicate the few residual and latent metastatic cells. These therapeutic regimens, however, are currently designed without direct information about the presence or nature of the latent cells. To address this problem, we developed a PCR-based technique to analyze the transcriptome of individual tumor cells isolated from the bone marrow of cancer patients. From the same cells, genomic aberrations were identified by comparative genomic hybridization. The utility of this approach for understanding the biology of occult disseminated cells and for the identification of new therapeutic targets is demonstrated here by the detection of frequent extracellular matrix metalloproteinase inducer (EMMPRIN; CD147) expression which was verified by immunostaining.

References

Jan 1, 1979·Methods in Enzymology·T Nelson, D Brutlag
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G SchlimokG Riethmüller
Apr 25, 1989·Nucleic Acids Research·A BelyavskyK Rajewsky
Mar 15, 1988·International Journal of Cancer. Journal International Du Cancer·H W Ziegler-HeitbrockG Riethmüller
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·E E KarrerD Alexander
Jan 1, 1993·Methods in Enzymology·G Brady, N N Iscove
Oct 4, 1996·Cell·P A Baeuerle, D Baltimore
Dec 6, 1996·Science·C J Sherr
Feb 1, 1997·Current Opinion in Cell Biology·N Tapon, A Hall
May 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M PoletteP Birembaut
Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·P A AndreasenM J Duffy
Jun 26, 1998·The Biochemical Journal·J Gomez-Marquez, P Rodríguez
Jan 12, 1999·Nature Medicine·L LuoM G Erlander
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·C A KleinG Riethmüller
Jul 7, 1999·Journal of the National Cancer Institute·K PantelO Fodstad
Oct 16, 1999·International Journal of Cancer. Journal International Du Cancer·A SerranoT Boon
Nov 5, 1999·Nature Biotechnology·M Mahadevappa, J A Warrington
Dec 3, 1999·Science·T Lindahl, R D Wood
Oct 26, 2000·Trends in Cell Biology·C A Klein

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Citations

Oct 8, 2005·Pflügers Archiv : European journal of physiology·Guylaine M DurandArthur Konnerth
Sep 7, 2011·Breast Cancer Research and Treatment·Brant PohorelicA Magliocco
Jun 1, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Kai BartkowiakKlaus Pantel
Mar 9, 2005·Trends in Cell Biology·Weigang WangJohn S Condeelis
Dec 9, 2003·The Breast : Official Journal of the European Society of Mastology·Stephan BraunKlaus Pantel
Dec 14, 2002·Trends in Cell Biology·Jeffrey M Levsky, Robert H Singer
Dec 10, 2002·Current Opinion in Chemical Biology·Wenonah Vercoutere, Mark Akeson
Jun 18, 2002·Trends in Molecular Medicine·Randy Todd, David H Margolin
May 19, 2009·Molecular Pharmaceutics·Priya BalasubramanianJeffrey J Chalmers
Jun 24, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Wulf SienelChristoph A Klein
Apr 1, 2011·Nature Methods·Fuchou TangM Azim Surani
Mar 6, 2010·Nature Protocols·Fuchou TangM Azim Surani
Jul 2, 2004·Nature Reviews. Cancer·Bryan P Toole
Mar 25, 2009·Nature Reviews. Cancer·Christoph A Klein
Jul 31, 2013·Nature Reviews. Genetics·Ehud ShapiroSten Linnarsson
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hidetoshi MoriCeleste M Nelson
Nov 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ichiro OtaStephen J Weiss
Oct 31, 2006·Nucleic Acids Research·Claudia H Hartmann, Christoph A Klein
Sep 20, 2006·Genes & Development·Kevin HotaryStephen J Weiss
Jun 3, 2004·Plant Physiology·Blake C MeyersVikas Agrawal
Dec 14, 2007·Journal of Virology·Oliver FrankWolfgang Seifarth
Apr 4, 2012·Annual Review of Chemical and Biomolecular Engineering·Frederik S O FritzschAndreas Schmid
Jul 19, 2008·Annual Review of Analytical Chemistry·Daniella CohenNorman J Dovichi
Jan 10, 2008·Annual Review of Cell and Developmental Biology·R Grant Rowe, Stephen J Weiss
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mark A WatsonRebecca L Aft
Mar 24, 2005·BMC Cell Biology·Sarah L Windler-HartAnjen Chenn
Feb 19, 2013·BMC Molecular Biology·Christopher J WeltyColm Morrissey
Jun 15, 2013·BMC Cancer·Frank A W CoumansLeon W M M Terstappen
Jan 20, 2010·Breast Cancer Research : BCR·Sabine Riethdorf, Klaus Pantel
Mar 6, 2012·Journal of Cell Science·G Daniel GrassBryan P Toole
Oct 3, 2006·PLoS Genetics·Carlos A RamosMargaret A Goodell
Jun 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Oleg Schmidt-KittlerChristoph A Klein
Jul 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Robert T KrivacicRichard H Bruce
Dec 3, 2013·Analytical and Bioanalytical Chemistry·Thomas HaselgrüblerJan Hesse

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