Combined transgenic expression of Δ12-desaturase and Δ12-epoxygenase in high linoleic acid seeds leads to increased accumulation of vernolic acid

Functional Plant Biology : FPB
Xue-Rong ZhouAllan G Green

Abstract

The transgenic production of unusual fatty acids in oil seed crops offers an alternative, renewable resource for industry. However, transgenic expression of genes catalysing the synthesis of unusual fatty acids has generally resulted in these fatty acids accumulating at levels significantly below the levels in the wild species from which the genes were sourced. This study reports expression of additional copies of any of three Δ12-desaturase genes (FAD2) from Crepis palaestina Bornm., cotton (Gossypium hirsutum L.) or Arabidopsis thaliana (L.) Heynh. with C. palaestina Δ12-epoxygenase gene (Cpal2), in an Arabidopsis mutant having a significantly higher level of linoleic acid substrate. This resulted in the highest levels of vernolic acid accumulation, 21% of total fatty acids, reported so far in any transgenic plant expressing the Δ12-epoxygenase. Similarly, the co-expression of C. palaestina Cpal2 and a transgenic copy of FAD2 in cotton seed that contains large amounts of linoleic acid substrate also resulted in greater accumulation of vernolic acid in seed than did expression of C. palaestina Cpal2 alone.

References

Jan 1, 1980·Progress in Lipid Research·R C Badami, K B Patil
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·F J van de LooC Somerville
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·E B CahoonA J Kinney
Mar 9, 2000·Trends in Plant Science·A A MillarL Kunst
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Toni Voelker, Anthony J Kinney
Jan 22, 2002·Metabolic Engineering·Jay J Thelen, John B Ohlrogge
Aug 15, 2002·Plant Physiology·Peter A StoutjesdijkAllan G Green
Apr 2, 2003·Current Opinion in Plant Biology·Jan Jaworski, Edgar B Cahoon
Jan 21, 2004·Phytochemistry·Olga V Sayanova, Johnathan A Napier
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sandra TrenkampKlaus Tietjen
Mar 9, 2005·Current Opinion in Plant Biology·Surinder P SinghAllan G Green
Jul 1, 2005·Functional Plant Biology : FPB·Stan S RobertAllan G Green

❮ Previous
Next ❯

Citations

Jul 28, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Elizabeth S DennisW J Peacock
Jan 19, 2010·Plant Biotechnology Journal·Runzhi LiDavid F Hildebrand
May 3, 2007·Annual Review of Plant Biology·Johnathan A Napier
May 3, 2011·Transgenic Research·James R PetrieSurinder P Singh
May 23, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Qing LiuSurinder Singh
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·John M DyerAnders S Carlsson
Aug 26, 2020·Metabolic Engineering·Yang XuGuanqun Chen

❮ Previous
Next ❯

Datasets Mentioned

BETA
X97016

Methods Mentioned

BETA
transgenic
PCR
phosphotransferase
transgenically

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.