Combined use of AFM and soft X-ray microscopy to reveal fibres' internalization in mesothelial cells

The Analyst
Alessandra GianoncelliLorella Pascolo

Abstract

Nanotoxicology and nanomedicine investigations often require the probing of nano-objects such as fibres and particles in biological samples and cells, whilst internalization and intracellular destiny are the main issues for in vitro cellular studies. Various high resolution microscopy techniques are well suited for providing this highly sought-after information. However, sample preparation, nanomaterial composition and sectioning challenges make it often difficult to establish whether the fibres or particles have been internalized or they are simply overlaying or underlying the biological matter. In this paper we suggest a novel suitable combination of two different microscopic techniques to reveal in intact cells the uptake of asbestos fibres by mesothelial cells. After exposure to asbestos fibres and fixation, cells were first analysed under the AFM instrument and then imaged under the TwinMic soft X-ray microscope at Elettra Sincrotrone. The suggested approach combines standard soft X-ray microscopy imaging and AFM microscopy, with a common non-invasive sample preparation protocol which drastically reduces the experimental uncertainty and provides a quick and definitive answer to the nanoparticle cellular and tissue uptake.

References

Feb 1, 1995·Quarterly Reviews of Biophysics·J KirzM Howells
Mar 24, 1999·Trends in Cell Biology·C Jacobsen
Aug 9, 2008·International Journal of Nanomedicine·Wim H De Jong, Paul J A Borm
May 7, 2009·The Lancet Oncology·Kurt StraifUNKNOWN WHO International Agency for Research on Cancer Monograph Working Group
Jul 3, 2009·Journal of the Royal Society, Interface·Burkhard KaulichMaya Kiskinova
Apr 30, 2010·Proceedings of the American Thoracic Society·James C Bonner
May 28, 2013·ACS Nano·Claudia GottsteinJoseph Anthony Zasadzinski
Oct 28, 2016·Journal of Synchrotron Radiation·Alessandra GianoncelliAnna Bianco

❮ Previous
Next ❯

Citations

Jun 11, 2019·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Takenori IshidaAkio Kuroda
Oct 14, 2021·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Akio Kuroda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Yi-Gang YuYi-Quan Ke
Journal of the American Medical Association
O DRESSLER
Annals of the New York Academy of Sciences
M S Badollet, W A Gantt
© 2022 Meta ULC. All rights reserved