Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity

Conservation Biology : the Journal of the Society for Conservation Biology
Michael StatEuan S Harvey

Abstract

Monitoring communities of fish is important for the management and sustainability of fisheries and marine ecosystems. Baited remote underwater video systems (BRUVs) are among the most effective nondestructive techniques for sampling bony fishes and elasmobranchs (sharks, rays, and skates). However, BRUVs sample visually conspicuous biota; hence, some taxa are undersampled or not recorded at all. We compared the diversity of fishes characterized using BRUVs with diversity detected via environmental DNA (eDNA) metabarcoding. We sampled seawater and captured BRUVs imagery at 48 locales that included reef and seagrass beds inside and outside a marine reserve (Jurien Bay in Western Australia). Eighty-two fish genera from 13 orders were detected, and the community of fishes described using eDNA and BRUVs combined yielded >30% more generic richness than when either method was used alone. Rather than detecting a homogenous genetic signature, the eDNA assemblages mirrored the BRUVs' spatial explicitness; differentiation of taxa between seagrass and reef was clear despite the relatively small geographical scale of the study site (∼35 km2 ). Taxa that were not sampled by one approach, due to limitations and biases intrinsic to the method,...Continue Reading

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Citations

Oct 22, 2019·Molecular Ecology Resources·Girish KumarMichelle R Gaither
Jul 9, 2020·Proceedings. Biological Sciences·Jean-Baptiste JuhelRégis Hocdé
Oct 21, 2020·Molecular Ecology·Giorgio AglieriMarco Milazzo
Oct 16, 2020·The Science of the Total Environment·Shuping WangJianlong Li
Dec 9, 2020·Ecological Applications : a Publication of the Ecological Society of America·Tommaso RussoStefano Mariani
Jul 6, 2021·Conservation Biology : the Journal of the Society for Conservation Biology·Virginie MarquesSébastien Villéger
Nov 19, 2021·The Science of the Total Environment·Morgan R EllisCraig D H Sherman
Jan 18, 2022·The Science of the Total Environment·Steen Wilhelm KnudsenJesper H Andersen

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Methods Mentioned

BETA
PCR

Software Mentioned

JBMP
R R Development Core Team
PRIMER
PERMANOVA
blastn
EventMeasure ( Stereo )
SIMPER
Geneious
vegan community ecology package
Xilisoft Video Converter Ultimate

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