Combined use of salivary biomarkers and carcinoembryonic antigen for lung cancer detection in a Chinese population

Medicine
Xi ShangYoumin Zhang

Abstract

Blood-based biomarkers, such as carcinoembryonic antigen (CEA), and saliva-based biomarkers, such as mRNA, have emerged as potential liquid biopsies for non-invasive detection of many cancers. However, current tests typically use single type of biomarkers, and their sensitivity and specificity is often unsatisfactory.In this study, we developed a novel biomarker panel that measures both CEA level in blood and GREB1 and FRS2 levels in saliva to achieve high sensitivity and high specificity in detecting Non-Small Cell Lung Cancer (NSCLC).In the discovery phase, we achieved sensitivity of 96.67% and specificity of 93.33% for 30 NSCLC patients and 30 healthy controls. To further evaluate the prediction performance of our biomarker panel, we applied it to an independent set of 15 NSCLC cancer patients and 25 healthy controls. The sensitivity and specificity of our test reached 93.33% and 80.00% respectively.Our study discovered that the combined analysis of CEA and mRNA can be a novel liquid-biopsy technology for non-invasive detection of NSCLC.

References

Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Avrum SpiraJerome S Brody
Aug 18, 2004·Archives of Otolaryngology--head & Neck Surgery·Maie A R St JohnDavid T W Wong
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yang LiDavid T Wong
Aug 21, 2009·Nature Reviews. Cancer·Prabhat Jha
Aug 27, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Noh Jin ParkDavid T Wong
Feb 19, 2011·Journal of Biomedicine & Biotechnology·Akira Mogi, Hiroyuki Kuwano
Nov 19, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yu-Hsiang LeeDavid T Wong
Feb 24, 2012·Molecular Oncology·N Lynn Henry, Daniel F Hayes
Jun 13, 2012·Cellular and Molecular Life Sciences : CMLS·Lei ZhangDavid T W Wong
Jun 14, 2014·Journal of the National Comprehensive Cancer Network : JNCCN·Anya LitvakLeonard Saltz
Mar 10, 2015·Computational and Structural Biotechnology Journal·Konstantina KourouDimitrios I Fotiadis
Sep 4, 2015·Cancer·Qun-Ying HongUNKNOWN Lung Cancer Group of Chinese Thoracic Society; Chinese Alliance Against Lung Cancer
Feb 6, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Frederico Omar Gleber-NettoDavid T Wong
Nov 4, 2016·The Oncologist·Pascale TomasiniNatasha B Leighl
Jul 2, 2017·Pathology·Yamato MotookaTakaaki Ito
Sep 22, 2017·PloS One·Ehsan SaghapourMohammadreza Sehhati
Nov 8, 2017·Journal of Proteome Research·Fadhl M AlakwaaLana X Garmire
Nov 25, 2017·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Yoko NakanishiShu Hashimoto
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Apr 22, 2018·Biochemical and Biophysical Research Communications·Toshiyuki IshibaKathleen Danenberg

❮ Previous
Next ❯

Citations

Nov 17, 2019·International Journal of Molecular Sciences·Heng Liang Tan, Andre Choo
Aug 29, 2020·Journal of Clinical Medicine·Alena LiskovaOlga Golubnitschaja
Jun 19, 2020·Healthcare·Antonio Sanchez-FernandezMaría Pedrera-Canal

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

ARCHITECT
CancerSEEK
Statistics and Machine Learning Toolbox
Statistics Machine Learning Toolbox
MATLAB
MedCalc

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.