Combined use of the modified Hodge test and carbapenemase inhibition test for detection of carbapenemase-producing Enterobacteriaceae and metallo-β-lactamase-producing Pseudomonas spp

Annals of Laboratory Medicine
Wonkeun SongIl Kwon Bae

Abstract

We evaluated the combined use of the modified Hodge test (MHT) and carbapenemase inhibition test (CIT) using phenylboronic acid (PBA) and EDTA to detect carbapenemase-producing Enterobacteriaceae (CPE) and metallo-β-lactamase (MBL)-producing Pseudomonas spp. A total of 49 isolates of CPE (15 Klebsiella pneumoniae carbapenemase [KPC], 5 Guiana extended-spectrum β-lactamase [GES]-5, 9 New Delhi metallo-β-lactamase [NDM]-1, 5 Verona integron-encoded metallo-β-lactamase [VIM]-2, 3 imipenem-hydrolyzing β-lactamase [IMP], and 12 oxacillinase [OXA]-48-like), 25 isolates of MBL-producing Pseudomonas spp. (14 VIM-2 and 11 IMP), and 35 carbapenemase-negative controls were included. The MHT was performed for all isolates as recommended by the Clinical and Laboratory Standards Institute. Enhanced growth of the indicator strain was measured in mm with a ruler. The CIT was performed by directly dripping PBA and EDTA solutions onto carbapenem disks that were placed on Mueller-Hinton agar plates seeded with the test strain. Considering the results of the MHT with the ertapenem disk in Enterobacteriaceae and Pseudomonas spp., the CIT with the meropenem disk in Enterobacteriaceae, and the imipenem disk in Pseudomonas spp., three combined disk te...Continue Reading

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Citations

Dec 8, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G MikucionyteA Oliver
Mar 16, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Tanise Vendruscolo DalmolinMarli Matiko Anraku de Campos
Oct 9, 2015·Annals of Clinical Microbiology and Antimicrobials·Kazım SahinEmine Sonmez
Jun 24, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H SolgiF Shahcheraghi
Oct 3, 2018·Journal of Korean Medical Science·Jin-Hong Yoo
Sep 15, 2020·International Journal of Microbiology·Benjelloun Touimi GhitaBahia Bennani
Jun 18, 2021·Antimicrobial Resistance and Infection Control·Priscila Pinho da SilvaUNKNOWN Nucleus of Hospital Research Study Collaborators
Dec 16, 2021·Annals of Laboratory Medicine·Inho Choi, Yangsoon Lee

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Methods Mentioned

BETA
PCR

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