Combining 25-Hydroxycholesterol with an HIV Fusion Inhibitor Peptide: Interaction with Biomembrane Model Systems and Human Blood Cells

ACS Infectious Diseases
Bárbara GomesNuno C Santos

Abstract

The fusion between the viral and the target cell membrane is a crucial step in the life cycle of enveloped viruses. The blocking of this process is a well-known therapeutic approach that led to the development of the fusion inhibitor peptide enfuvirtide, clinically used against human immunodeficiency virus (HIV) type 1. Despite this significant advance on viral treatment, the appearance of resistance has limited its clinical use. Such a limitation has led to the development of other fusion inhibitor peptides, such as C34, that present the same structural domain as enfuvirtide (heptad repeat sequence) but have different functional domains (pocket-binding domain in the case of C34 and lipid-binding domain in the case of enfuvirtide). Recently, the antiviral properties of 25-hydroxycholesterol were demonstrated, which boosted the interest in this oxysterol. The combination of two distinct antiviral molecules, C34 and 25-hydroxycholesterol, may help to suppress the emergence of resistant viruses. In this work, we characterized the interaction of the C34-25-hydroxycholesterol conjugate with biomembrane model systems and human blood cells. Lipid vesicles and monolayers with defined lipid compositions were used as biomembrane model sy...Continue Reading

References

Aug 1, 1988·Journal of Virological Methods·R PauwelsE De Clercq
Jul 7, 1988·Biochimica Et Biophysica Acta·J F Nagle, M C Wiener
Jun 13, 1986·Biochimica Et Biophysica Acta·L D MayerP R Cullis
Jan 31, 1997·Biochimica Et Biophysica Acta·R J Clarke, D J Kane
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·D C ChanP S Kim
Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
Nov 9, 2000·Genes & Development·R W Doms, D Trono
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·D M Eckert, P S Kim
Jul 10, 2002·Lancet·Christoph HessJürg-Alfred Schifferli
May 21, 2003·Nature Reviews. Drug Discovery·Jason LaBontePeter Kirkpatrick
Jun 6, 2003·Biochimica Et Biophysica Acta·Nuno C SantosMiguel A R B Castanho
Jul 23, 2003·Biochimica Et Biophysica Acta·Stephen A GalloRobert Blumenthal
Aug 5, 2003·Antiviral Research·Mercedes Armand-UgónJosé A Esté
Nov 13, 2004·Journal of the American Chemical Society·A Salomé VeigaMiguel A R B Castanho
Mar 8, 2006·Steroids·Emily J Westover, Douglas F Covey
Jun 2, 2006·Chemistry and Physics of Lipids·Alexander I GreenwoodJohn F Nagle
Jul 4, 2008·Nature Structural & Molecular Biology·Stephen C Harrison
Aug 30, 2008·Biochimica Et Biophysica Acta·Katharina LeidlGerd Schmitz
Mar 20, 2009·Proceedings of the National Academy of Sciences of the United States of America·Paolo IngallinellaAntonello Pessi
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·David R BaumanDavid W Russell
Dec 17, 2009·PloS One·Zoltan BeckCarl R Alving
Dec 25, 2009·Current Pharmaceutical Design·Imke Steffen, Stefan Pöhlmann
Apr 2, 2010·Journal of Virology·Matteo PorottoAnne Moscona
Jul 22, 2010·Biochimica Et Biophysica Acta·Pedro M MatosNuno C Santos
Nov 16, 2010·Biochemical and Biophysical Research Communications·Pedro M MatosNuno C Santos
Dec 21, 2011·Antiviral Chemistry & Chemotherapy·Fusako Miyamoto, Eiichi N Kodama
Aug 22, 2012·Cold Spring Harbor Perspectives in Medicine·Craig B WilenRobert W Doms
Oct 9, 2012·The Journal of Biological Chemistry·Robert BlumenthalMathias Viard

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Marco M DominguesNuno C Santos
Sep 4, 2021·Biochimica Et Biophysica Acta. Biomembranes·Patrícia M SilvaNuno C Santos

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