Combining a nontargeted and targeted metabolomics approach to identify metabolic pathways significantly altered in polycystic ovary syndrome

Metabolism: Clinical and Experimental
Alice Y ChangK Sreekumaran Nair

Abstract

Polycystic ovary syndrome (PCOS) is a condition of androgen excess and chronic anovulation frequently associated with insulin resistance. We combined a nontargeted and targeted metabolomics approach to identify pathways and metabolites that distinguished PCOS from metabolic syndrome (MetS). Twenty obese women with PCOS were compared with 18 obese women without PCOS. Both groups met criteria for MetS but could not have diabetes mellitus or take medications that treat PCOS or affect lipids or insulin sensitivity. Insulin sensitivity was derived from the frequently sampled intravenous glucose tolerance test. A nontargeted metabolomics approach was performed on fasting plasma samples to identify differentially expressed metabolites, which were further evaluated by principal component and pathway enrichment analysis. Quantitative targeted metabolomics was then applied on candidate metabolites. Measured metabolites were tested for associations with PCOS and clinical variables by logistic and linear regression analyses. This multiethnic, obese sample was matched by age (PCOS, 37±6; MetS, 40±6years) and body mass index (BMI) (PCOS, 34.6±5.1; MetS, 33.7±5.2kg/m2). Principal component analysis of the nontargeted metabolomics data showed ...Continue Reading

Citations

Feb 13, 2019·American Journal of Physiology. Endocrinology and Metabolism·Melanie Cree-GreenKristen J Nadeau
Jun 1, 2018·Clinical Chemistry·John W Honour
Jan 5, 2019·Current Medicinal Chemistry·Igor GovorovEduard Komlichenko
Mar 16, 2019·Frontiers in Endocrinology·Anna HalamaStephen L Atkin
Jul 29, 2020·Human Molecular Genetics·Jake P MannClaudia Langenberg
Apr 2, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Zhexin NiChaoqin Yu
May 5, 2021·Seminars in Reproductive Medicine·Simon AlesiAya Mousa
Oct 21, 2021·The Journal of Clinical Endocrinology and Metabolism·Tiantian Zhu, Mark O Goodarzi

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