Combining chemical and genetic approaches to increase drought resistance in plants

Nature Communications
Minjie CaoJian-Kang Zhu

Abstract

Drought stress is a major threat to crop production, but effective methods to mitigate the adverse effects of drought are not available. Here, we report that adding fluorine atoms in the benzyl ring of the abscisic acid (ABA) receptor agonist AM1 optimizes its binding to ABA receptors by increasing the number of hydrogen bonds between the compound and the surrounding amino acid residues in the receptor ligand-binding pocket. The new chemicals, known as AMFs, have long-lasting effects in promoting stomatal closure and inducing the expression of stress-responsive genes. Application of AMFs or transgenic overexpression of the receptor PYL2 in Arabidopsis and soybean plants confers increased drought resistance. The greatest increase in drought resistance is achieved when AMFs are applied to the PYL2-overexpression transgenic plants. Our results demonstrate that the combining of potent chemicals with transgenic overexpression of an ABA receptor is very effective in helping plants combat drought stress.

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Citations

Jul 10, 2018·Plant & Cell Physiology·Wim DejongheSean R Cutler
Oct 28, 2019·Science·Aditya S VaidyaSean R Cutler
Apr 4, 2019·Plant Molecular Biology·Seungsu HanSangho Lee
Mar 17, 2019·International Journal of Molecular Sciences·Yajun ChenLu Zhang
Oct 6, 2020·The Plant Journal : for Cell and Molecular Biology·Mingnan QuXinguang Zhu
Feb 3, 2021·Journal of Experimental Botany·Irene Garcia-MaquilonPedro L Rodriguez
Jun 3, 2021·Plants·Rafael Ruiz-PartidaJorge Lozano-Juste
Dec 18, 2018·Journal of Agricultural and Food Chemistry·Danying MaShiping Tian
Sep 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Aditya S VaidyaSean R Cutler
Sep 26, 2021·Nature Reviews. Genetics·Huiming ZhangJian-Kang Zhu
Dec 23, 2021·Physical Chemistry Chemical Physics : PCCP·Chuankai Zhao, Diwakar Shukla

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Datasets Mentioned

BETA
GSE101488

Methods Mentioned

BETA
transgenic
AlphaScreen
RNA-seq
chemical treatment
NMR
X-ray
PCR

Software Mentioned

DENZO
CNS
OpenCV
Phaser
Refmac5
LigPlot
R pheatmap
Coot
CCP4
TopHat

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