Combining chemoselective ligation with polyhistidine-driven self-assembly for the modular display of biomolecules on quantum dots.

ACS Nano
Duane E PrasuhnIgor L Medintz

Abstract

One of the principle hurdles to wider incorporation of semiconductor quantum dots (QDs) in biology is the lack of facile linkage chemistries to create different types of functional QD--bioconjugates. A two-step modular strategy for the presentation of biomolecules on CdSe/ZnS core/shell QDs is described here which utilizes a chemoselective, aniline-catalyzed hydrazone coupling chemistry to append hexahistidine sequences onto peptides and DNA. This specifically provides them the ability to ratiometrically self-assemble to hydrophilic QDs. The versatility of this labeling approach was highlighted by ligating proteolytic substrate peptides, an oligoarginine cell-penetrating peptide, or a DNA-probe to cognate hexahistidine peptidyl sequences. The modularity allowed subsequently self-assembled QD constructs to engage in different types of targeted bioassays. The self-assembly and photophysical properties of individual QD conjugates were first confirmed by gel electrophoresis and Forster resonance energy transfer analysis. QD-dye-labeled peptide conjugates were then used as biosensors to quantitatively monitor the proteolytic activity of caspase-3 or elastase enzymes from different species. These sensors allowed the determination of ...Continue Reading

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Citations

Jul 27, 2010·Analytical and Bioanalytical Chemistry·Anthony J TavaresUlrich J Krull
Dec 21, 2013·ACS Applied Materials & Interfaces·Jonathan C ClaussenIgor L Medintz
Jan 20, 2012·ACS Nano·Susan Buckhout-WhiteIgor L Medintz
Aug 24, 2012·Theranostics·Yi Zhang, Tza-Huei Wang
Oct 23, 2013·Analytical Biochemistry·Gwan-Ho LeeSang Soo Hah
Sep 25, 2012·Advanced Drug Delivery Reviews·Christine E ProbstXiaohu Gao
Mar 24, 2012·Chemical Communications : Chem Comm·Haiyan Zhang, Dejian Zhou
Jun 9, 2015·Sensors·Armen ShamirianPreston T Snee
May 13, 2014·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Amy M ScottIgor L Medintz
Sep 8, 2015·Advanced Drug Delivery Reviews·Daisuke OnoshimaYoshinobu Baba
Aug 27, 2014·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Joyce BregerIgor L Medintz
Nov 5, 2013·Trends in Biotechnology·Svetlana AvvakumovaDavide Prosperi
Jul 29, 2015·Analytical and Bioanalytical Chemistry·Sebastián A DíazIgor L Medintz
Oct 13, 2010·Nanoscale·Ashwinkumar BhirdeXiaoyuan Chen
Dec 4, 2013·Physical Chemistry Chemical Physics : PCCP·Christina Marie Tyrakowski, Preston Todd Snee
Feb 11, 2014·Analytical Chemistry·Christina M Tyrakowski, Preston T Snee
Dec 15, 2010·Journal of the American Chemical Society·Kelly BoenemanIgor L Medintz
Apr 11, 2017·Nanoscale·James N VranishIgor L Medintz
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Jan 25, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Jing WangWenying Zhong
May 1, 2013·Methods and Applications in Fluorescence·Alexander P Demchenko

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