Combining chitinase C and N-acetylhexosaminidase from Streptomyces coelicolor A3(2) provides an efficient way to synthesize N-acetylglucosamine from crystalline chitin

Journal of Biotechnology
Nhung Nguyen-Thi, Nicolas Doucet

Abstract

The enzymatic bioconversion of chitin is of considerable interest for the natural production of bioactive compounds such as chitooligosaccharides and N-acetyl-d-glucosamine (GlcNAc). Key enzymes are involved in the natural processing of chitin, hydrolyzing this abundant biopolymer to yield chitooligosaccharides with substantial value to the medicinal and biotechnological fields. In this study, chitinase C (ScChiC) from the soil bacterium and chitin decomposer Streptomyces coelicolor A3(2) was expressed, purified and characterized. We also optimized a Streptomyces lividans system generating ScChiC expression yields nearly 500-fold higher than the previously reported heterologous expression in Escherichia coli. The purified enzyme was found to be stable below 55°C for a broad range of pH values (pH 3.5-9) and exhibited high activity against chitin and chitooligosaccharides to form chitobiose (C2) as main product. Crab shell chitin hydrolysis profiles also revealed that ScChiC catalyzes the bioconversion of chitopolysaccharides through an endo-nonprocessive mode of action. When combining ScChiC with an N-acetylhexosaminidase from S. coelicolor A3(2) (ScHEX) in an assay using crude extracts and crystalline chitin as substrate, GlcN...Continue Reading

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Citations

Aug 28, 2016·Bioresource Technology·Papa Rao VaikuntapuAppa Rao Podile
Dec 13, 2016·Inorganic Chemistry·Xiu-Ying ZhengLan-Sun Zheng
Mar 5, 2018·Applied Microbiology and Biotechnology·Jelena SpasicJasmina Nikodinovic-Runic
Oct 29, 2017·World Journal of Microbiology & Biotechnology·Flávio Augusto CardozoIrma Nelly Gutierrez Rivera
Jul 26, 2018·Bioscience Reports·Ayokunmi Oyeleye, Yahaya M Normi
Jan 24, 2019·Bioprocess and Biosystems Engineering·Shun JiangGuimin Zhang
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Jul 25, 2019·World Journal of Microbiology & Biotechnology·Flávio Augusto CardozoAdalberto Pessoa
Sep 26, 2020·Frontiers in Microbiology·Francesca BeriniElisa Binda
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Huihui SunXiangzhao Mao
Jun 12, 2021·Current Opinion in Biotechnology·Jingyu ZhangXueting Liu
Apr 11, 2020·Journal of Agricultural and Food Chemistry·Tian LiuQing Yang

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