Combining complete chloroplast genome sequences with target loci data and morphology to resolve species limits in Triplostegia (Caprifoliaceae)

Molecular Phylogenetics and Evolution
Yan-Ting NiuZhi-Duan Chen

Abstract

Species represent the most basic unit of taxonomy. As such, species delimitation represents a crucial issue for biodiversity conservation. Taxonomic practices were revolutionized in the last three decades due to the increasing availability of molecular phylogenetic data. The genus Triplostegia (Caprifoliaceae) traditionally consists of two species, T. glandulifera and T. grandiflora, distinguishable mainly based on quantitative morphological features. In this study, we sequenced nine chloroplast loci (i.e., accD, psbK-psbI, rbcL-accD, rpoB-trnC, rps16-trnQ, trnE-trnT, trnF-ndhJ, trnH-psbA, trnS-trnG) and one nuclear locus (ITS) of 16 individuals of Triplostegia representing the entire distribution range of both species recognized. Furthermore, we also obtained whole chloroplast sequences for 11 of the 16 individuals for which silica gel-dried leaves were available. Our phylogenetic analyses integrating chloroplast genome sequences and multiple loci data revealed that Triplostegia includes four main clades that largely match geography. Neither T. grandiflora nor T. glandulifera was recovered as monophyletic and no diagnosable differences in leaf, flower, and pollen traits were detected between the two species, indicating the nee...Continue Reading

References

Feb 5, 2003·Molecular Phylogenetics and Evolution·Wen-Heng ZhangYan-Cheng Tang
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Jun 8, 2004·Bioinformatics·Stacia K WymanJeffrey L Boore
Jun 25, 2004·Nucleic Acids Research·Kelly A FrazerInna Dubchak
Jul 6, 2004·The Quarterly Review of Biology·Paul-Michael AgapowAndy Purvis
Jul 5, 2005·Science
Nov 21, 2007·Systematic Biology·Kevin De Queiroz
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Robert K JansenJeffrey L Boore
Mar 20, 2008·Genome Research·Daniel R Zerbino, Ewan Birney
Oct 15, 2008·Systematic Biology·Alexandros StamatakisJacques Rougemont
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·UNKNOWN CBOL Plant Working Group
Aug 1, 2006·Journal of Mammalogy·Robert J Baker, Robert D Bradley
Aug 28, 2010·Plant Biotechnology Journal·Catherine J NockRobert J Henry
Apr 6, 2011·Evolution; International Journal of Organic Evolution·Bernhard Hausdorf
May 27, 2011·American Journal of Botany·Douglas E SoltisPamela S Soltis
Feb 24, 2012·Systematic Biology·Fredrik RonquistJohn P Huelsenbeck
May 29, 2012·Trends in Ecology & Evolution·Matthew K FujitaCraig Moritz
Aug 1, 2012·Nature Methods·Diego DarribaDavid Posada
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Nov 28, 2013·Annual Review of Genetics·Mizuki TakenakaAxel Brennicke
Mar 29, 2014·Biological Reviews of the Cambridge Philosophical Society·Xiwen LiShilin Chen
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Jan 6, 2016·Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis·Guo-Qing BaiSi-Feng Li
Jun 25, 2016·Genome Biology·Henry DaniellWan-Jung Chang
Jun 13, 2017·Molecular Phylogenetics and Evolution·J C ZimmersJ F Smith

❮ Previous
Next ❯

Citations

Sep 17, 2019·PhytoKeys·Catalina Ruiz-DomínguezVictoria Sosa
May 30, 2020·International Journal of Molecular Sciences·Zhou HongDaping Xu
Oct 13, 2019·Molecular Phylogenetics and Evolution·Hong-Xin WangHua-Feng Wang
May 11, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Bruno Cesar RossiniCelso Luis Marino
May 17, 2021·BMC Plant Biology·Xiao-Feng ZhangHua-Feng Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.