Combining desorption electrospray ionization mass spectrometry and nuclear magnetic resonance for differential metabolomics without sample preparation

Rapid Communications in Mass Spectrometry : RCM
Huanwen ChenR Graham Cooks

Abstract

Desorption electrospray ionization mass spectrometry (DESI-MS) and nuclear magnetic resonance (NMR) spectroscopy are used to provide data on urine examined without sample preparation to allow differentiation between diseased (lung cancer) and healthy mice. Principal component analysis (PCA) is used to shortlist compounds with potential for biomarker screening which are responsible for significant differences between control urine samples and samples from diseased animals. Similar PCA score plots have been achieved by DESI-MS and NMR, using a subset of common detected metabolites. The common compounds detected by DESI and NMR have the same changes in sign of their concentrations thereby indicating the usefulness of corroborative analytical methods. The effects of different solvents and surfaces on the DESI mass spectra are also evaluated and optimized. Over 80 different metabolites were successfully identified by DESI-MS and tandem mass spectrometry experiments, with no prior sample preparation.

References

Sep 1, 1997·Journal of Neurochemistry·K TakahashiT Nishikawa
Jun 23, 2000·Enzyme and Microbial Technology·M Farfán, I L Calderón
Aug 21, 2001·Chemical Research in Toxicology·A W NichollsJ K Nicholson
Jul 18, 2002·Nature Reviews. Drug Discovery·Jeremy K NicholsonElaine Holmes
Oct 16, 2002·Drug and Chemical Toxicology·Nicholas V Reo
Aug 2, 2003·Science·Toni Gabaldón, Martijn A Huynen
Oct 18, 2003·Journal of Biomedical Optics·Michael D KennedyPhilip S Low
Dec 17, 2003·Analytical Chemistry·Helena Idborg-BjörkmanSven P Jacobsson
Jun 16, 2004·Current Opinion in Microbiology·Douglas B Kell
Jun 19, 2004·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·John C LindonJeremy K Nicholson
Sep 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Daniel CookMichael F Thomashow
Sep 29, 2004·Current Opinion in Chemical Biology·Jan van der GreefRob van der Heijden
Nov 17, 2004·Current Drug Metabolism·J L Griffin, M E Bollard
Feb 12, 2005·The Journal of Biological Chemistry·Mingzhu LiuFrederick R Blattner
Mar 19, 2005·Electrophoresis·Philippe Schmitt-Kopplin, Matthias Englmann
Apr 15, 2005·Analytical Chemistry·Robert B CodyH Dupont Durst
Apr 27, 2005·The Analyst·Warwick B DunnHelen E Johnson
Jul 15, 2005·Analytical Chemistry·Li YangMatthew R Linford
Nov 16, 2005·Rapid Communications in Mass Spectrometry : RCM·Jonathan P Williams, James H Scrivens
Nov 22, 2005·Rapid Communications in Mass Spectrometry : RCM·Jentaie ShieaJan Sunner
Dec 7, 2005·Rapid Communications in Mass Spectrometry : RCM·Luc Alexis LeutholdGérard Hopfgartner
Mar 18, 2006·Science·R Graham CooksJustin M Wiseman

❮ Previous
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Citations

Sep 7, 2006·Analytical and Bioanalytical Chemistry·Zhengzheng Pan, Daniel Raftery
Mar 12, 2009·Analytical and Bioanalytical Chemistry·Leonard NyadongFacundo M Fernández
Jan 18, 2013·Analytical and Bioanalytical Chemistry·Lukas VaclavikJana Hajslova
Jun 1, 2006·Journal of the American Society for Mass Spectrometry·Marcela NefliuColin Moore
Nov 6, 2007·Journal of the American Society for Mass Spectrometry·Ayanna U JacksonGary J Van Berkel
Jan 12, 2008·Journal of the American Society for Mass Spectrometry·Marcela NefliuR Graham Cooks
Jan 12, 2008·Journal of the American Society for Mass Spectrometry·Hong ChenJian-Min Chen
Aug 8, 2008·Journal of the American Society for Mass Spectrometry·Joseph E Chipuk, Jennifer S Brodbelt
Dec 30, 2008·Journal of the American Society for Mass Spectrometry·Joseph E Chipuk, Jennifer S Brodbelt
Mar 31, 2007·Analytical Chemistry·Yong-Seung ShinFranco Basile
Mar 20, 2013·Chemical Reviews·Naomi L Kuehnbaum, Philip Britz-McKibbin
Sep 27, 2007·Chemical Communications : Chem Comm·Anthony B Costa, R Graham Cooks
Dec 8, 2010·Neurosurgery·Nathalie Y R AgarFerenc A Jolesz
May 19, 2010·Biomarkers in Medicine·G A Nagana GowdaTedros Bezabeh
Jul 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Narasimhamurthy ShanaiahDaniel Raftery
Nov 6, 2014·Analytical and Bioanalytical Chemistry·Josep GómezNicolau Cañellas
Jun 6, 2008·Expert Review of Proteomics·Isabelle FournierMichel Salzet
Mar 2, 2012·Expert Review of Gastroenterology & Hepatology·Neeral R PatelSimon D Taylor-Robinson
Sep 13, 2008·Expert Review of Molecular Diagnostics·G A Nagana GowdaDaniel Raftery
Mar 25, 2010·Expert Opinion on Drug Discovery·Robert Powers
Dec 23, 2015·Analytical Chemistry·Andreas SchedlAntje Potthast
Dec 14, 2011·Analytica Chimica Acta·Georgios A TheodoridisIan D Wilson
Dec 21, 2010·Analytica Chimica Acta·D RyanMegan Kendall
May 20, 2009·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Nicholas J ZyromskiDaniel Raftery
Jan 20, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Allison L DillR Graham Cooks
Aug 2, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Erwan WernerJean-Claude Tabet
Aug 21, 2007·Journal of Pharmaceutical and Biomedical Analysis·Haiwei GuDaniel Raftery
May 15, 2009·NMR in Biomedicine·Haiwei GuDaniel Raftery
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Feb 14, 2007·Journal of Mass Spectrometry : JMS·Guodong ChenUrooj A Mirza
Apr 4, 2007·Mass Spectrometry Reviews·Xuewu ZhangFeng Chen
May 13, 2008·Journal of Mass Spectrometry : JMS·Chris Petucci, Jason Diffendal
Jul 19, 2008·Mass Spectrometry Reviews·Xiaojun FengBi-Feng Liu
Nov 1, 2007·Rapid Communications in Mass Spectrometry : RCM·Gaetano CorsoOceania D'Apolito

❮ Previous
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