Combining High-Throughput Sequencing of sedaDNA and Traditional Paleolimnological Techniques To Infer Historical Trends in Cyanobacterial Communities

Environmental Science & Technology
Timothy J TsePaul D Jones

Abstract

Freshwaters worldwide are under increasing pressure from anthropogenic activities and changing climate. Unfortunately, many inland waters lack sufficient long-term monitoring to assess environmental trends. Analysis of sedimentary ancient DNA ( sedaDNA) is emerging as a means to reconstruct the past occurrence of microbial communities of inland waters. The purpose of this study was to assess a combination of high-throughput sequencing (16S rRNA) of sedaDNA and traditional paleolimnological analyses to explore multidecadal relationships among cyanobacterial community composition, the potential for cyanotoxin production, and paleoenvironmental proxies. DNA was extracted from two sediment cores collected from a northern Canadian Great Plains reservoir. Diversity indices illustrated significant community-level changes since reservoir formation. Furthermore, higher relative abundances in more recent years were observed for potentially toxic cyanobacterial genera including Dolichospermum. Correlation-based network analysis revealed this trend significantly and positively correlated to abundances of the microcystin synthetase gene ( mcyA) and other paleoproxies (nutrients, pigments, stanols, sterols, and certain diatom species), demon...Continue Reading

References

Aug 1, 1997·Applied and Environmental Microbiology·U NübelG Muyzer
Oct 31, 1998·Applied and Environmental Microbiology·M J Coolen, J Overmann
Jun 14, 2003·TheScientificWorldJournal·H W PaerlJ Dyble
Oct 11, 2003·Archives of Microbiology·Michael HisberguesThomas Börner
Jan 9, 2004·Current Biology : CB·Eske WillerslevAlan Cooper
Nov 18, 2005·Journal of Microbiological Methods·C BoutteA Wilmotte
Jan 10, 2006·BMC Bioinformatics·Ramón Díaz-Uriarte, Sara Alvarez de Andrés
Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mitchell L SoginGerhard J Herndl
Jan 16, 2007·Applied Microbiology and Biotechnology·Martin L SakerVitor M Vasconcelos
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Apr 10, 2008·Environmental Microbiology·Marco J L CoolenJaap S Sinninghe Damsté
Aug 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·David W SchindlerS E M Kasian
Feb 21, 2009·Science·Daniel J ConleyGene E Likens
Mar 25, 2011·The ISME Journal·Joshua A SteeleJed A Fuhrman
Sep 2, 2011·The ISME Journal·Alexander EilerStefan Bertilsson
Sep 9, 2011·The ISME Journal·Albert BarberánNoah Fierer
Apr 11, 2012·Molecular Ecology·Shadi ShokrallaMehrdad Hajibabaei
Dec 25, 2012·Applied and Environmental Microbiology·Virginia LozaPilar Mateo
Apr 17, 2013·Molecular Ecology·Laura ParducciEske Willerslev
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Marco J L CoolenLiviu Giosan
Oct 26, 2013·Science·Hans W Paerl, Timothy G Otten
Aug 6, 2016·Environmental Science & Technology·David W SchindlerDiane M Orihel
Aug 28, 2016·Applied and Environmental Microbiology·Marie-Eve MonchampPiet Spaak
Dec 13, 2017·Nature Ecology & Evolution·Marie-Eve MonchampFrancesco Pomati

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ancient DNA

Ancient DNA sequences are able to offer valuable insights into molecular evolutionary processes, but are notoriously difficult to analyze due to molecular damage and exogenous dna contamination. Discover the latest research on Ancient DNA here.

© 2022 Meta ULC. All rights reserved