Combining liquid chromatography with ozone-induced dissociation for the separation and identification of phosphatidylcholine double bond isomers

Analytical and Bioanalytical Chemistry
Rachel L KozlowskiStephen J Blanksby

Abstract

Revealing the inherent molecular diversity of lipid biology requires advanced analytical technologies. Distinguishing phospholipids that differ in the position(s) of carbon-carbon double bonds within their acyl chains presents a particular challenge because of their similar chromatographic and mass spectral behaviours. Here-for the first time-we combine reversed-phase liquid chromatography for separation of isomeric phospholipids with on-line mass spectral analysis by ozone-induced dissociation (OzID) for unambiguous double bond position assignment. The customised tandem linear ion-trap mass spectrometer used in our study is capable of acquiring OzID scans on a chromatographic timescale. Resolving the contributions of isomeric lipids that are indistinguishable based on conventional mass spectral analysis is achieved using the combination of liquid chromatography and OzID. Application of this method to the analysis of simple (egg yolk) and more complex (sheep brain) extracts reveals significant populations of the phosphatidylcholine PC 16:0_18:1(n-7) alongside the expected PC 16:0_18:1(n-9) isomer.

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Citations

Feb 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xiaoxiao MaYu Xia
Sep 22, 2016·Analytical Biochemistry·Sarah E HancockTodd W Mitchell
Nov 22, 2017·PloS One·Josch K PaulingChrister S Ejsing
Jun 19, 2018·Current Opinion in Clinical Nutrition and Metabolic Care·Juan J Aristizabal HenaoKen D Stark
Dec 14, 2017·Journal of the American Society for Mass Spectrometry·Craig A StinsonYu Xia
Sep 13, 2019·Journal of Lipid Research·Sarah E HancockTodd W Mitchell
Dec 24, 2018·Analytical and Bioanalytical Chemistry·Katharina WoznyBritta Brügger
Mar 16, 2021·Analytical Chemistry·Marcos BouzaFacundo M Fernández
Aug 24, 2017·Journal of the American Society for Mass Spectrometry·Rodell C BarrientosQibin Zhang

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