Combining MALDI-FTMS and bioinformatics for rapid peptidomic comparisons

Journal of Proteome Research
Joshua J SchmidtLingjun Li

Abstract

Increasing research efforts in large-scale mass spectral analyses of peptides and proteins have led to many advances in technology and method development for collecting data and improving the quality of data. However, the resultant large data sets often pose significant challenges in extracting useful information in a high-throughput manner. Here, we describe one such method where we analyzed a large mass spectral data set collected using decapod crustacean nervous tissue extracts separated via high-performance liquid chromatography (HPLC) coupled to high-resolution matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS). Following their acquisition, the data collected from discrete LC fractions was compiled and analyzed using an in-house developed software package that deisotoped, compressed, calibrated, and matched peaks to a list of known crustacean neuropeptides. By processing these data via bioinformatics tools such as hierarchical clustering, more than 110 neuropeptides that belong to 14 peptide families were mapped in five crustacean species. Overall, we demonstrate the utility of MALDI-FTMS in combination with a bioinformatics software package for the elucidation and comparison of pe...Continue Reading

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Citations

Aug 21, 2010·Cellular and Molecular Life Sciences : CMLS·Andrew E ChristiePatsy S Dickinson
Feb 5, 2015·Food Science & Nutrition·Tim T LambersEric Af van Tol
Dec 22, 2014·Biochimica Et Biophysica Acta·Wouter De HaesKurt Boonen
Nov 10, 2020·Mass Spectrometry Reviews·Kellen DeLaneyLingjun Li

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