Combining Molecular Weight Fractionation and Metabolomics to Elucidate the Bioactivity of Vegetal Protein Hydrolysates in Tomato Plants

Frontiers in Plant Science
Luigi LuciniGiuseppe Colla

Abstract

The comprehension of the bioactive fractions involved in the biostimulant activity of plant derived protein hydrolysates (PH) is a complex task, but it can also lead to significant improvements in the production of more effective plant biostimulants. The aim of this work is to shed light onto the bioactivity of different PH dialysis fractions (PH1 < 0.5-1 kDa; PH2 > 0.5-1 kDa; PH3 < 8-10 kDa; PH4 > 8-10 kDa) of a commercial PH-based biostimulant through a combined in vivo bioassay and metabolomics approach. A first tomato rooting bioassay investigated the auxin-like activity of PH and its fractions, each of them at three nitrogen levels (3, 30, and 300 mg L-1 of N) in comparison with a negative control (water) and a positive control (indole-3-butyric acid, IBA). Thereafter, a second experiment was carried out where metabolomics was applied to elucidate the biochemical changes imposed by the PH and its best performing fraction (both at 300 mg L-1 of N) in comparison to water and IBA. Overall, both the PH and its fractions increased the root length of tomato cuttings, compared to negative control. Moreover, the highest root length was obtained in the treatment PH1 following foliar application. Metabolomics allowed highlighting a ...Continue Reading

References

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Jan 10, 2018·Frontiers in Plant Science·Giuseppe CollaYoussef Rouphael
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Dec 4, 2019·Food Chemistry·Enrique Barrajón-CatalánVicente Micol

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Citations

Jun 3, 2021·Biomolecules·Mohamad Hesam ShahrajabianSpyridon A Petropoulos
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Amna Ben HassineSafia El-Bok
Aug 28, 2021·Biomolecules·Miguel BaltazarBerta Gonçalves

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Methods Mentioned

BETA
Profiler
metabolomic profiling

Software Mentioned

Agilent Profinder
PlantCyc
DA
PlantCyc Pathway Tool
SPSS
COSMOS
SIMCA
OPLS
PlantCyc pathway Tools
Pathway Tools Omics Dashboard

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