Combining naproxen and a dual amylin and calcitonin receptor agonist improves pain and structural outcomes in the collagen-induced arthritis rat model

Arthritis Research & Therapy
Anna KatriKim Henriksen

Abstract

Pain is a debilitating symptom of rheumatoid arthritis (RA), caused by joint inflammation and cartilage and bone destruction. Nonsteroidal anti-inflammatory drugs (NSAIDs) are used to treat pain and inflammation in RA, but are not disease-modifying and do not prevent joint destruction when administered alone. KBPs (Key Bioscience peptides) are synthetic peptides based on salmon calcitonin and are expected to inhibit bone resorption and to be chondroprotective. In this study, we investigated if combining a standard of care NSAID (naproxen) with a KBP resulted in improvement in pain scores, as well as disease activity and structural damage in a rat model of RA. Collagen-induced arthritis (CIA) was induced in 40 female Lewis rats by immunization with porcine type II collagen; 10 rats were given sham injections. CIA rats were treated with KBP and/or naproxen. Health scores and joint scores were evaluated daily. Mechanical and cold allodynia tests and burrowing tests were used to assess pain-like behaviors. Blood samples were collected for biomarker testing, and paws were collected for histology and microcomputed tomography. Naproxen monotherapy increased the time until humane endpoints was reached, and improved health score, pain a...Continue Reading

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Citations

Dec 5, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·David N PagliaJ Patrick O'Connor
Apr 28, 2021·Molecular Medicine·Li-Rong RenKai-Shun Yang

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad Prism
Olympus cellSens

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