Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics

Archives of Biochemistry and Biophysics
Haydyn D T Mertens, Dmitri I Svergun

Abstract

Small-angle X-ray scattering (SAXS) and Nuclear Magnetic Resonance (NMR) are established methods to analyze the structure and structural transitions of biological macromolecules in solution. Both methods are directly applicable to near-native macromolecular solutions and allow one to study structural responses to physical and chemical changes or ligand additions. Whereas SAXS is applied to elucidate overall structure, interactions and flexibility over a wide range of particle sizes, NMR yields atomic resolution detail for moderately sized macromolecules. NMR is arguably the most powerful technique for the experimental analysis of dynamics. The joint application of these two highly complementary techniques provides an extremely useful approach that facilitates comprehensive characterization of biomacromolecular solutions.

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Citations

Dec 24, 2018·RNA·Meghan S WardenSteven M Pascal
Jan 14, 2021·Analytical Chemistry·Yunfei HuMaili Liu
Apr 20, 2018·Emerging Topics in Life Sciences·Martin A Schroer, Dmitri I Svergun
Apr 10, 2021·Journal of Applied Crystallography·Karen Manalastas-CantosDaniel Franke
Apr 27, 2021·ChemCatChem·Pratul K AgarwalNicolas Doucet
Jul 19, 2021·Journal of Molecular Graphics & Modelling·Melanie SchneiderGilles Labesse

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Methods Mentioned

BETA
NMR
electron
X-ray
small-angle scattering
dynamic light scattering

Software Mentioned

SASREF
CRYSOL
pyDockSAXS
SHANUM
SASFLOW
AUTORG
ATSAS
BUNCH
Xplor
AUTOGNOM

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