Abstract
Nerve tissue engineering is an important strategy for the treatment of brain injuries. Mesenchymal stem cell (MSC) transplantation has been proven to be able to promote repair and functional recovery of brain damage, and poly (lactic-co-glycolic acid) (PLGA) has also been found to have the capability of bearing cells. In the present study, to observe the ability of PLGA scaffold in supporting the adherent growth of MSCs and neurons in vivo and vitro and to assess the effects of PLGA scaffold on proliferation and neural differentiation of MSCs, this study undertakes the following steps. First, MSCs and neurons were cultured and labeled with green fluorescent protein (GFP) or otherwise identified and the PLGA scaffold was synthesized. Next, MSCs and neurons were inoculated on PLGA scaffolds and their adhesion rates were investigated and the proliferation of MSCs was evaluated by using MTT assay. After MSCs were induced by a neural induction medium, the morphological change and neural differentiation of MSCs were detected using scanning electron microscopy (SEM) and immunocytochemistry, respectively. Finally, cell migration and adhesion in the PLGA scaffold in vivo were examined by immunohistochemistry, nuclear staining, and SEM. ...Continue Reading
References
Mar 11, 2000·Biomaterials·K Anselme
Mar 8, 2005·Stem Cells·Kyung Jin ChoPranela Rameshwar
Dec 7, 2007·Acta Biomaterialia·Xigeng MiaoRoss Crawford
Feb 12, 2011·Neurosurgery·Chaoxian YangQionglan Yuan
Sep 10, 2011·Tissue Engineering. Part B, Reviews·Regis J O'Keefe, Jeremy Mao
Mar 30, 2012·Birth Defects Research. Part C, Embryo Today : Reviews·Nianli Zhang, David H Kohn
Jul 25, 2012·Differentiation; Research in Biological Diversity·Jessian L MunozAllan Siegel
Nov 5, 2013·Neurochemical Research·Yu ZengZhixiong Liu
Apr 11, 2014·BioMed Research International·Adnan HaiderInn-Kyu Kang
Feb 28, 2015·The Journal of Craniofacial Surgery·Li JunyiJiang Yan
Jun 10, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·C FornagueraC Solans
Feb 6, 2016·International Journal of Nanomedicine·Bor-Shiunn LeeYi-Wen Chen
Jul 16, 2016·Biological Chemistry·Safoura KhajeniaziAnvarsadat Kianmehr
Oct 19, 2016·Acta Biomaterialia·W ShiJ Chen
Sep 13, 2016·Journal of Neuroscience Research·Dandan ChenXin Wang
Nov 11, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Rio KurimotoRyuji Kato
Nov 28, 2016·Pharmacology & Therapeutics·Elisa AvolioPaola Campagnolo
May 10, 2017·Matrix Biology : Journal of the International Society for Matrix Biology·Uma Thanigai ArasuKirsi Rilla
Jun 21, 2017·Stem Cells International·Jing Qu, Huanxiang Zhang
Nov 7, 2017·Cell Journal·Rozmehr ShokohiJaleel A Miyan
Nov 15, 2017·Histochemistry and Cell Biology·Joanna StacheckaIzabela Szczerbal
Dec 20, 2017·Molecular Medicine Reports·Akira NemotoAkira Ishisaki
Citations
Oct 4, 2020·Pharmaceutics·Gillian D MahumaneYahya E Choonara
Feb 14, 2020·Biomedical Materials·Madhumita PatelWon-Gun Koh
Jul 28, 2020·Cell and Tissue Research·Sajad Sahab NegahAli Gorji
Apr 9, 2020·World Journal of Stem Cells·Matteo ScopettiVittorio Fineschi
Aug 28, 2021·International Journal of Molecular Sciences·Teruki Nii, Yoshiki Katayama