Combining protein identification and quantification: C-terminal isotope-coded tagging using sulfanilic acid

Rapid Communications in Mass Spectrometry : RCM
Alexandre PanchaudMartin Kussmann

Abstract

Two methods of differential isotopic coding of carboxylic groups have been developed to date. The first approach uses d0- or d3-methanol to convert carboxyl groups into the corresponding methyl esters. The second relies on the incorporation of two 18O atoms into the C-terminal carboxylic group during tryptic digestion of proteins in H(2)18O. However, both methods have limitations such as chromatographic separation of 1H and 2H derivatives or overlap of isotopic distributions of light and heavy forms due to small mass shifts. Here we present a new tagging approach based on the specific incorporation of sulfanilic acid into carboxylic groups. The reagent was synthesized in a heavy form (13C phenyl ring), showing no chromatographic shift and an optimal isotopic separation with a 6 Da mass shift. Moreover, sulfanilic acid allows for simplified fragmentation in matrix-assisted laser desorption/ionization (MALDI) due the charge fixation of the sulfonate group at the C-terminus of the peptide. The derivatization is simple, specific and minimizes the number of sample treatment steps that can strongly alter the sample composition. The quantification is reproducible within an order of magnitude and can be analyzed either by electrospray ...Continue Reading

References

Oct 1, 1994·Rapid Communications in Mass Spectrometry : RCM·I LindhJ Sjövall
Apr 29, 2000·Electrophoresis·G L CorthalsJ C Sanchez
Jul 11, 2001·Rapid Communications in Mass Spectrometry : RCM·D R GoodlettE Kolker
Nov 28, 2001·Analytical Chemistry·R ZhangF E Regnier
Dec 18, 2001·Rapid Communications in Mass Spectrometry : RCM·I I StewartD Figeys
May 10, 2002·Journal of Chromatography. a·Asish Chakraborty, Fred E Regnier
Aug 15, 2002·Analytical Chemistry·Roujian ZhangFred E Regnier
Mar 21, 2003·Journal of Proteome Research·Peiran Liu, Fred E Regnier
Nov 5, 2003·Proteomics·Bernd Moritz, Helmut E Meyer
Dec 13, 2003·Analytical Chemistry·Jue-Liang HsuShu-Hui Chen
Jul 16, 2004·Journal of Proteome Research·Samir Julka, Fred Regnier
Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alexander Leitner, Wolfgang Lindner

❮ Previous
Next ❯

Citations

May 3, 2008·Journal of the American Society for Mass Spectrometry·Hannah JohnsonSimon J Gaskell
Jul 26, 2011·The Proceedings of the Nutrition Society·Sandra Sénéchal, Martin Kussmann
Mar 24, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Martin Kussmann
Jun 11, 2008·Journal of Proteomics·Alexandre PanchaudMartin Kussmann
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Apr 8, 2006·Journal of Biotechnology·Martin KussmannMichael Affolter
Dec 18, 2007·Molecular & Cellular Proteomics : MCP·Alexandre PanchaudMartin Kussmann
Aug 17, 2006·Current Opinion in Clinical Nutrition and Metabolic Care·Martin Kussmann, Michael Affolter
Feb 22, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jiahe LuoZhengzhi Zeng
Oct 13, 2009·Journal of Proteome Research·Hong WangSamir Hanash

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.