Combining selection valve and mixing chamber for nanoflow gradient generation: Toward developing a liquid chromatography cartridge coupled with mass spectrometer for protein and peptide analysis

Analytica Chimica Acta
Apeng ChenShaorong Liu

Abstract

Toward developing a micro HPLC cartridge, we have recently built a high-pressure electroosmotic pump (EOP). However, we do not recommend people to use this pump to deliver an organic solvent directly, because it often makes the pump rate unstable. We have experimented several approaches to address this issue, but none of them are satisfactory. Here, we develop an innovative approach to address this issue. We first create an abruption (a dead-volume) within a fluid conduit. We then utilize an EOP to withdraw, via a selection valve, a train of eluent solutions having decreasing eluting power into the fluid conduit. When these solutions are further aspirated through the dead-volume, these solutions are partially mixed, smoothening concentration transitions between two adjacent eluent solutions. As these solutions are pushed back, through the dead-volume again, a smooth gradient profile is formed. In this work, we characterize this scheme for gradient formation, and we incorporate this approach with a high-pressure EOP, a nanoliter injection valve, and a capillary column, yielding a micro HPLC system. We then couple this micro HPLC with an electrospray ionization - mass spectrometer for peptide and protein separations and identific...Continue Reading

References

Jan 1, 1992·Rapid Communications in Mass Spectrometry : RCM·J ZaiaK Biemann
Jun 1, 1991·Protein Engineering·J G GuillemetteM Smith
Feb 28, 2004·Analytical Chemistry·Kisaburo DeguchiAkihiro Takeda
May 2, 2007·The Analyst·Fu-Qiang NieBrett Paull
Oct 31, 2009·Journal of Separation Science·María Asensio-RamosSalvatore Fanali
Nov 21, 2009·Journal of the American Chemical Society·Jun YiGeorge B Richter-Addo
Aug 3, 2010·The Journal of Experimental Biology·U B Hendgen-CottaT Rassaf
Jun 3, 2011·The Journal of Biological Chemistry·Jeremiah D TiptonNeil L Kelleher
Nov 11, 2011·Journal of Chromatography. a·Jörg P Kutter
Oct 16, 2012·Analytical Chemistry·Congying GuShaorong Liu
Jan 8, 2014·Disease Models & Mechanisms·Julie S Valastyan, Susan Lindquist

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