Combining the preparation of oligonucleotide arrays and synthesis of high-quality primers

Analytical Biochemistry
J Weiler, Jörg D Hoheisel

Abstract

Based on the oligomer-chip technology, oligonucleotide arrays were synthesized directly on polypropylene sheets by a modified phosphoramidite chemistry using beta-eliminating nucleobase-protecting groups in combination with a succinate solid-phase linker. This method decouples the oligonucleotide deprotection from the support cleavage procedure, in contrast to standard phosphoramidite chemistry. In addition to being reliable substrates for hybridization experiments, the arrays also serve as source for the isolation of individual oligonucleotides. Technically, this allowed for a direct control of the quality of the arrayed oligomers. The released compounds were sufficient in amount and purity to work without further purification in PCR and DNA-sequencing reactions, with the results being identical to controls with commercially obtained primer molecules. Consequences for oligomer-chip hybridization procedures, the applicability of such hybrid-function arrays in, for example, diagnostics or comparative biology, and developments toward parallel primer synthesis are discussed.

Citations

Jul 15, 1997·Nucleic Acids Research·J WeilerJ D Hoheisel
Feb 28, 1998·Nucleic Acids Research·C F EdmanM J Heller
Apr 13, 1999·Nucleic Acids Research·M Beier, J D Hoheisel
Jan 17, 2002·Journal of Biotechnology·Markus Beier, Jörg D Hoheisel
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·R T Pon
Feb 5, 2000·Nature Biotechnology·L WestinM Nerenberg
Jan 26, 2005·Chemistry : a European Journal·Bruno BujoliCharles Tellier
Jan 16, 1997·Nature·A Goffeau
Feb 11, 2010·Physical Chemistry Chemical Physics : PCCP·Vincenzo BaroneGiacomo Prampolini
Feb 18, 2006·Nature Reviews. Genetics·Jörg D Hoheisel
Mar 29, 2003·Prostate Cancer and Prostatic Diseases·F HamyT Klimkait
Jun 20, 2017·Current Protocols in Nucleic Acid Chemistry·Geeta MeherRadhakrishnan P Iyer
Feb 22, 2002·The Journal of Organic Chemistry·Richard T PonYogesh S Sanghvi
Feb 5, 2004·Journal of the American Chemical Society·Guillaume NonglatonBruno Bujoli

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