Combining Untargeted and Targeted Proteomic Strategies for Discrimination and Quantification of Cashmere Fibers

PloS One
Shanshan LiWenqing Shui

Abstract

Cashmere is regarded as a specialty and luxury fiber due to its scarcity and high economic value. For fiber quality assessment, it is technically very challenging to distinguish and quantify the cashmere fiber from yak or wool fibers because of their highly similar physical appearance and substantial protein sequence homology. To address this issue, we propose a workflow combining untargeted and targeted proteomics strategies for selecting, verifying and quantifying biomarkers for cashmere textile authentication. Untargeted proteomic surveys were first applied to identify 174, 157, and 156 proteins from cashmere, wool and yak fibers, respectively. After marker selection at different levels, peptides turned out to afford much higher selectivity than proteins for fiber species discrimination. Subsequently, parallel reaction monitoring (PRM) methods were developed for ten selected peptide markers. The PRM-based targeted analysis of peptide markers enabled accurate determination of fiber species and cashmere percentages in different fiber mixtures. Furthermore, collective use of these peptide makers allowed us to discriminate and quantify cashmere fibers in commercial finished fabrics that have undergone heavy chemical treatments. ...Continue Reading

References

Dec 9, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·R J McLarenG W Montgomery
Mar 31, 2005·International Review of Cytology·Lutz Langbein, Jürgen Schweizer
Aug 31, 2006·International Review of Cytology·Michael A RogersJürgen Schweizer
Oct 20, 2006·Kidney International·M Lewin, S Sethi
Mar 21, 2009·Nature Protocols·Paul J BoersemaAlbert J R Heck
Jun 10, 2009·Journal of Proteome Research·Je-Hyun BaekMyeong-Hee Yu
Apr 29, 2010·Journal of Proteome Research·Henning KoehnJeffrey E Plowman
May 19, 2010·Journal of Proteomics·Stefan ClerensJolon M Dyer
Dec 31, 2010·Forensic Science International : Synergy·Wan JiXue Yang
Mar 15, 2011·Molecular Biology Reports·Hua GongJon G H Hickford
Jul 21, 2011·Experimental Dermatology·Hua GongJon Hickford
Apr 7, 2012·Journal of Proteomics·Jeffrey E PlowmanJolon M Dyer
Aug 7, 2012·Molecular & Cellular Proteomics : MCP·Amelia C PetersonJoshua J Coon
Sep 12, 2013·Molecular & Cellular Proteomics : MCP·Adam D CathermanNeil L Kelleher
Apr 9, 2014·Journal of Proteomics·André Martinho AlmeidaStefan Clerens
May 30, 2014·Nature·Mathias WilhelmBernhard Kuster

❮ Previous
Next ❯

Citations

Sep 30, 2017·Proteomics. Clinical Applications·Henry A AdeolaNonhlanhla P Khumalo
Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jifeng LiZhaofang Du
Apr 18, 2019·PeerJ·So Young Ryu, George A Wendt

❮ Previous
Next ❯

Datasets Mentioned

BETA
PXD003107

Methods Mentioned

BETA
optical
scanning electron microscopy
chemical treatments
chemical treatment
light microscopy

Software Mentioned

Excel
SIMCA
Mascot
ProteinPilot TM
ProteinPilot
mProphet
Skyline

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.