Combining Whole-Genome Sequencing and Multimodel Phenotyping To Identify Genetic Predictors of Salmonella Virulence.

MSphere
Alanna CrouseFrance Daigle

Abstract

Salmonella comprises more than 2,600 serovars. Very few environmental and uncommon serovars have been characterized for their potential role in virulence and human infections. A complementary in vitro and in vivo systematic high-throughput analysis of virulence was used to elucidate the association between genetic and phenotypic variations across Salmonella isolates. The goal was to develop a strategy for the classification of isolates as a benchmark and predict virulence levels of isolates. Thirty-five phylogenetically distant strains of unknown virulence were selected from the Salmonella Foodborne Syst-OMICS (SalFoS) collection, representing 34 different serovars isolated from various sources. Isolates were evaluated for virulence in 4 complementary models of infection to compare virulence traits with the genomics data, including interactions with human intestinal epithelial cells, human macrophages, and amoeba. In vivo testing was conducted using the mouse model of Salmonella systemic infection. Significant correlations were identified between the different models. We identified a collection of novel hypothetical and conserved proteins associated with isolates that generate a high burden. We also showed that blind prediction...Continue Reading

References

Jan 1, 1990·Microbial Pathogenesis·P A Gulig
Apr 1, 2000·Microbes and Infection·S L MarcusB B Finlay
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jun 9, 2004·Applied and Environmental Microbiology·Dilek Tezcan-MerdolMikael Rhen
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hervé TettelinClaire M Fraser
Sep 24, 2005·Infection and Immunity·Kaoru GeddesFred Heffron
Oct 20, 2006·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Gabriel G PerronGraham Bell
Nov 14, 2007·The Journal of Experimental Medicine·Marie-France RoyDanielle Malo
Jan 7, 2009·Journal of Bacteriology·Javier F Mariscotti, Francisco García-del Portillo
Feb 18, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shannon E MajowiczUNKNOWN International Collaboration on Enteric Disease 'Burden of Illness' Studies
Mar 13, 2010·PloS One·Morgan N PriceAdam P Arkin
Aug 3, 2010·Journal of Bacteriology·Carol FarrisSamuel I Miller
Feb 24, 2011·Infection and Immunity·Renée M TsolisAndreas J Bäumler
Sep 20, 2011·Applied and Environmental Microbiology·Frédéric Douesnard-Malo, France Daigle
Jun 28, 2012·PLoS Pathogens·Mark AchtmanUNKNOWN S. Enterica MLST Study Group
Sep 12, 2013·PloS One·María de Las Mercedes PescarettiMónica A Delgado
Dec 18, 2013·Cell Host & Microbe·Carsten KrögerJay C D Hinton
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Gemma C LangridgeNicholas R Thomson
Jan 29, 2019·Frontiers in Microbiology·Angelina F A KuijpersAnnemarie Pielaat

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Citations

Dec 24, 2021·Molecular Microbiology·Adrian HerodJohn Rohde

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Datasets Mentioned

BETA
PRJNA634019

Methods Mentioned

BETA
PCA
phosphotransferase

Software Mentioned

MUSCLE
multcomp
Ade4
FastTree
nlme
BMGE
SaturnV
perl
R
Prokka

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