Combining Zebrafish and CRISPR/Cas9: Toward a More Efficient Drug Discovery Pipeline

Frontiers in Pharmacology
Carles CornetJavier Terriente

Abstract

The use of zebrafish larvae in basic and applied research has grown exponentially during the last 20 years. The reasons for this success lay in its specific experimental advantages: on the one hand, the small size, the large number of progeny and the fast life cycle greatly facilitate large-scale approaches while maintaining 3Rs amenability; on the other hand, high genetic and physiological homology with humans and ease of genetic manipulation make zebrafish larvae a highly robust model for understanding human disease. Together, these advantages allow using zebrafish larvae for performing high-throughput research, both in terms of chemical and genetic phenotypic screenings. Therefore, the zebrafish larva as an animal model is placed between more reductionist in vitro high-throughput screenings and informative but low-throughput preclinical assays using mammals. However, despite its biological advantages and growing translational validation, zebrafish remains scarcely used in current drug discovery pipelines. In a context in which the pharmaceutical industry is facing a productivity crisis in bringing new drugs to the market, the combined advantages of zebrafish and the CRISPR/Cas9 system, the most powerful technology for genomi...Continue Reading

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Jul 31, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Sylvia DyballaJavier Terriente
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Methods Mentioned

BETA
genotyping
transgenic

Software Mentioned

IDAA TM
TIDE

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