Combover interacts with the axonemal component Rsp3 and is required for Drosophila sperm individualization

Development
Josefa SteinhauerAndreas Jenny

Abstract

Gamete formation is key to survival of higher organisms. In male animals, spermatogenesis gives rise to interconnected spermatids that differentiate and individualize into mature sperm, each tightly enclosed by a plasma membrane. In Drosophila melanogaster, individualization of sister spermatids requires the formation of specialized actin cones that synchronously move along the sperm tails, removing inter-spermatid bridges and most of the cytoplasm. Here, we show that Combover (Cmb), originally identified as an effector of planar cell polarity (PCP) under control of Rho kinase, is essential for sperm individualization. cmb mutants are male sterile, with actin cones that fail to move in a synchronized manner along the flagella, despite being correctly formed and polarized initially. These defects are germline autonomous, independent of PCP genes, and can be rescued by wild-type Cmb, but not by a version of Cmb in which known Rho kinase phosphorylation sites are mutated. Furthermore, Cmb binds to the axonemal component Radial spoke protein 3, knockdown of which causes similar individualization defects, suggesting that Cmb coordinates the individualization machinery with the microtubular axonemes.

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D LuckB Huang
Jan 1, 1972·Zeitschrift für Zellforschung und mikroskopische Anatomie·K T TokuyasuR W Hardy
Jan 1, 1972·Zeitschrift für Zellforschung und mikroskopische Anatomie·K T TokuyasuR W Hardy
Jan 1, 1981·The Journal of Cell Biology·B HuangD J Luck
Apr 20, 2001·The Journal of Cell Biology·A R GaillardW S Sale
Nov 15, 2002·Journal of Cell Science·Aaron D Rogat, Kathryn G Miller
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Kirsi Riento, Anne J Ridley
Jun 26, 2003·Evolution & Development·Christopher Bazinet, Janet E Rollins
Mar 17, 2004·Molecular Biology of the Cell·Anindya Ghosh-RoyKrishanu Ray
Aug 30, 2005·Developmental Biology·Valerie MermallMark S Mooseker
Mar 2, 2006·Journal of Cell Science·Pinfen YangGeorge B Witman
Mar 31, 2006·Molecular Biology of the Cell·Tatsuhiko NoguchiKathryn G Miller
Oct 20, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jacques A VillefrancNathan D Lawson
Dec 25, 2007·Cell Motility and the Cytoskeleton·Maureen WirschellWinfield S Sale
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Aug 18, 2009·The Journal of Biological Chemistry·Arif JivanMelanie H Cobb
Aug 31, 2010·Cytoskeleton·Mutsuki AmanoKozo Kaibuchi
Jun 15, 2011·Cold Spring Harbor Perspectives in Biology·Michael P GreenbaumMartin M Matzuk
Oct 11, 2011·Organogenesis·Saw Myat Thanda W Maung, Andreas Jenny
Oct 23, 2012·Spermatogenesis·Lacramioara Fabian, Julie A Brill
Jan 30, 2014·Journal of Visualized Experiments : JoVE·Poojitha SitaramLaura Anne Lee
Apr 17, 2014·Seminars in Cell & Developmental Biology·Xiaojing QianC Yan Cheng
Sep 11, 2014·PloS One·Jeremy K FaganAndreas Jenny
Jun 18, 2015·Histochemistry and Cell Biology·Runchuan YanShanting Zhao
Sep 29, 2015·Spermatogenesis·Geulah Ben-DavidJosefa Steinhauer
Apr 26, 2016·Seminars in Cell & Developmental Biology·Haiqi Chen, C Yan Cheng
Jan 12, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·N A Siddall, G R Hime

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