Comet-FISH with rDNA probes for the analysis of mutagen-induced DNA damage in plant cells

Environmental and Molecular Mutagenesis
Jolanta KwasniewskaBozena Kolano

Abstract

We used comet-fluorescence in situ hybridization (FISH) in the model plant species Crepis capillaris following exposure of seedlings to maleic hydrazide (MH). FISH with 5S and 25S rDNA probes was applied to comets obtained under alkaline conditions to establish whether these DNA regions were preferentially involved in comet tail formation. MH treatment induced significant fragmentation of nuclear DNA and of rDNA loci. A 24-h post-treatment recovery period allowed a partial reversibility of MH-induced damage on nuclear and rDNA regions. Analyses of FISH signals demonstrated that rDNA sequences were always involved in tail formation and that 5S rDNA was more frequently present in the tail than 25S rDNA, regardless of treatment. The involvement of 25S rDNA in nucleolus formation and differences in chromatin structure between the two loci may explain the different susceptibility of the 25S and 5S rDNA regions to migrate into the tail. This work is the first report on the application of FISH to comet preparations from plants to analyze the distribution and repair of DNA damage within specific genomic regions after mutagenic treatment. Moreover, our work suggests that comet-FISH in plants may be a useful tool for environmental monito...Continue Reading

References

May 1, 1994·Bulletin of Environmental Contamination and Toxicology·D FairbairnK O'Neill
Jun 12, 1996·Mutation Research·M KlaudeG Ahnström
May 1, 1997·Experimental Cell Research·S J SantosA T Natarajan
Apr 29, 1997·Mutation Research·A R CollinsR Stĕtina
Jun 19, 1998·Mutation Research·W F Grant, E T Owens
Oct 9, 1999·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·M B MontijnN Nanninga
Jan 5, 2000·Environmental and Molecular Mutagenesis·S Cotelle, J F Férard
Mar 11, 2000·Environmental and Molecular Mutagenesis·M MenkeI Schubert
Nov 18, 2000·Journal of Photochemistry and Photobiology. B, Biology·A RappK O Greulich
Aug 31, 2006·Mutagenesis·Sergey ShaposhnikovMats Nilsson
Nov 18, 2006·Genome Génome / Conseil National De Recherches Canada·Jiming Jiang, Bikram S Gill
Feb 20, 2008·Mutagenesis·Andrew R CollinsRudolf Stetina
Feb 23, 2008·Mutation Research·Graciela SpivakPhilip C Hanawalt
Feb 29, 2008·Mutation Research·Michael GleiBeatrice L Pool-Zobel
Jun 26, 2008·Electrophoresis·Sergey A ShaposhnikovAndrew R Collins
Dec 8, 2010·Journal of Applied Genetics·Jolanta Juchimiuk-KwasniewskaJolanta Maluszynska
Feb 18, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Kendra L Cann, Graham Dellaire
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·J S Pat Heslop-Harrison, Trude Schwarzacher

❮ Previous
Next ❯

Citations

Oct 2, 2013·Journal of Applied Genetics·Jolanta Kwasniewska, Miroslaw Kwasniewski
Jan 30, 2014·PloS One·Jolanta Kwasniewska, Agnieszka Mikolajczyk
Aug 2, 2019·Plant Signaling & Behavior·Elif ÇakmakYıldız Aydın
Oct 3, 2020·Environmental Science and Pollution Research International·Cláudia CastroJosé Lima-Brito
Nov 18, 2020·International Journal of Molecular Sciences·Joanna JaskowiakIwona Szarejko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.