Commensal-derived OMVs elicit a mild proinflammatory response in intestinal epithelial cells

Microbiology
Daniel A PattenAndrew Collett

Abstract

Under normal physiological conditions, the intestinal immunity remains largely hyporesponsive to the commensal microbiota, yet also retains the inherent ability to rapidly respond to pathogenic antigens. However, immunomodulatory activities of extracellular products from commensal bacteria have been little studied, with previous investigations generally utilizing the live bacterium to study microbiota-epithelial interactions. In this study, we demonstrate that extracellular products of a commensal bacterium, Escherichia coli C25, elicit a moderate release of proinflammatory IL-8 and stimulate transcriptional up-regulation of Toll-like receptors (TLRs) in intestinal epithelial cell lines HT29-19A and Caco-2. Additionally, we show that removal of outer membrane vesicles (OMVs) reduces the proinflammatory effect of secreted products from E. coli C25. Furthermore, we show that isolated OMVs have a dose-dependent proinflammatory effect on intestinal epithelial cells (IECs). Interestingly, a relatively high concentration (40 µg ml-1 protein) of OMVs had no significant regulatory effects on TLR mRNA expression in both cell lines. Finally, we also demonstrate that pre-incubation with E. coli C25-derived OMVs subsequently inhibited the ...Continue Reading

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Citations

Jun 19, 2020·Journal of Diabetes and Metabolic Disorders·Ava BehrouziSeyed Davar Siadat
Mar 17, 2021·Molecular Immunology·William J GilmoreMaria Kaparakis-Liaskos
Aug 23, 2019·Comparative Immunology, Microbiology and Infectious Diseases·Fereshteh SatarianSeyed Davar Siadat
May 25, 2021·Frontiers in Cellular and Infection Microbiology·Nader KameliFrank R M Stassen
Jun 29, 2021·Frontiers in Pharmacology·Kanchana K Ayyar, Alan C Moss
Sep 3, 2021·Journal of Physiology and Biochemistry·Ester Díez-SainzSilvia Lorente-Cebrián
Oct 13, 2021·Trends in Immunology·Varnesh Tiku, Man-Wah Tan

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