Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water"

Science
Robert B BestBenjamin Schuler

Abstract

Riback et al (Reports, 13 October 2017, p. 238) used small-angle x-ray scattering (SAXS) experiments to infer a degree of compaction for unfolded proteins in water versus chemical denaturant that is highly consistent with the results from Förster resonance energy transfer (FRET) experiments. There is thus no "contradiction" between the two methods, nor evidence to support their claim that commonly used FRET fluorophores cause protein compaction.

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Citations

Jun 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ivan PeranDaniel P Raleigh
Sep 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Utsab R ShresthaLoukas Petridis
Apr 18, 2019·Proceedings of the National Academy of Sciences of the United States of America·Joshua A RibackTobin R Sosnick
Mar 17, 2019·The Journal of Chemical Physics·James LincoffTeresa Head-Gordon
Nov 11, 2018·Biomolecules·Sharonda J LeBlancKeith R Weninger
Dec 6, 2019·Current Opinion in Structural Biology·Robert B Best
Mar 17, 2021·The Journal of Physical Chemistry. B·William A Eaton
Apr 21, 2021·The Journal of Physical Chemistry. B·Thomas Dannenhoffer-Lafage, Robert B Best
Mar 28, 2019·The Journal of Physical Chemistry. B·Upayan BaulD Thirumalai
Oct 3, 2018·The Journal of Physical Chemistry. B·Wenwei ZhengRobert B Best
Aug 26, 2020·Journal of the American Chemical Society·Gregory-Neal W GomesClaudiu C Gradinaru

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