Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water".

Science
Gustavo FuertesEdward A Lemke

Abstract

Editors at Science requested our input on the above discussion (comment by Best et al and response by Riback et al) because both sets of authors use our data from Fuertes et al (2017) to support their arguments. The topic of discussion pertains to the discrepant inferences drawn from SAXS versus FRET measurements regarding the dimensions of intrinsically disordered proteins (IDPs) in aqueous solvents. Using SAXS measurements on labeled and unlabeled proteins, we ruled out the labels used for FRET measurements as the cause of discrepant inferences between the two methods. Instead, we propose that FRET and SAXS provide complementary readouts because of a decoupling of size and shape fluctuations that is intrinsic to finite-sized, heteropolymeric IDPs. Accounting for this decoupling resolves the discrepant inferences between the two methods, thus making a case for the utility of both methods.

References

Apr 2, 2009·The Journal of Chemical Physics·Edward P O'BrienD Thirumalai
Oct 30, 2014·Nucleic Acids Research·Erica ValentiniDmitri I Svergun
May 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Herschel M WatkinsKevin W Plaxco
Sep 2, 2016·Journal of the American Chemical Society·Alessandro BorgiaBenjamin Schuler
Jul 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Gustavo FuertesEdward A Lemke

❮ Previous
Next ❯

Citations

Apr 18, 2019·Proceedings of the National Academy of Sciences of the United States of America·Joshua A RibackTobin R Sosnick
Mar 17, 2019·The Journal of Chemical Physics·James LincoffTeresa Head-Gordon
Jun 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ivan PeranDaniel P Raleigh
Nov 11, 2018·Biomolecules·Sharonda J LeBlancKeith R Weninger
Dec 6, 2019·Current Opinion in Structural Biology·Robert B Best
Apr 9, 2021·Methods : a Companion to Methods in Enzymology·Jhullian J AlstonAlex S Holehouse
Mar 28, 2019·The Journal of Physical Chemistry. B·Upayan BaulD Thirumalai
Oct 3, 2018·The Journal of Physical Chemistry. B·Wenwei ZhengRobert B Best
Aug 26, 2020·Journal of the American Chemical Society·Gregory-Neal W GomesClaudiu C Gradinaru

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.