Commercial human albumin preparations for clinical use are immunosuppressive in vitro

Critical Care Medicine
David Bar-OrRichard Shimonkevitz

Abstract

We previously reported significant variations in oxidation status and molecular length among sources and lots of human serum albumin (HSA) commercial preparations intended for clinical use. In this report, we investigated what effect the presence of HSA products have on the immune response in vitro. Laboratory study. Trauma research basic science laboratory. Activated human peripheral blood mononuclear cells. Six commercial HSA preparations were tested for their effect on cytokine release from activated human peripheral blood mononuclear cells (PBMCs) and T-lymphocytes. Mass spectrometry analysis of aspartyl-alanyl diketopiperazine (DA-DKP) content of HSA and percentage of HSA having lost its amino terminal dipeptide aspartyl alanyl (HSA-DA) were correlated. Human PBMCs were cultured in the presence of six commercial HSA preparations and activated via the T-cell receptor complex. A cloned T-lymphocyte cell line, activated with specific antigen, was also cultured with both synthetic DA-DKP and small molecular weight extracts from the commercial HSA tested. Supernatants were quantified by enzyme-linked immunosorbent assay for interferon-gamma and tumor necrosis factor-alpha content. DA-DKP was extracted from HSA by centrifugal fi...Continue Reading

References

Jan 1, 1988·Neuroscience and Biobehavioral Reviews·C Prasad
Feb 1, 1983·The Journal of Clinical Endocrinology and Metabolism·Y TakaharaA Peterkofsky
Sep 1, 1994·International Journal of Peptide and Protein Research·J E BattersbyB O'Connor
Jan 15, 1995·European Journal of Biochemistry·B ChanR Harris
Apr 2, 1998·Journal of Pharmaceutical Sciences·C Goolcharran, R T Borchardt
Jul 24, 1998·BMJ : British Medical Journal·UNKNOWN Cochrane Injuries Group Albumin Reviewers
May 30, 2001·Critical Care Medicine·I von Hoegen, C Waller
Aug 23, 2002·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Shan-Yang LinTing-Chou Hu
Apr 20, 2004·Critical Care Medicine·Gregory J QuinlanTimothy W Evans
May 28, 2004·The New England Journal of Medicine·Simon FinferUNKNOWN SAFE Study Investigators
Nov 26, 2004·American Journal of Respiratory and Critical Care Medicine·UNKNOWN American Thoracic Society
Jul 9, 2005·Critical Care Medicine·David Bar-OrMichael L Craun

❮ Previous
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Citations

Apr 11, 2007·Critical Care Medicine·Christian J Wiedermann
Feb 19, 2013·Annals of Intensive Care·Myriam TavernaBertrand Guidet
Apr 2, 2009·Biological & Pharmaceutical Bulletin·Masaki Otagiri, Victor Tuan Giam Chuang
Aug 11, 2007·Expert Opinion on Drug Safety·Byron ChalidisPeter V Giannoudis
May 12, 2015·Journal of Immunoassay & Immunochemistry·Gregory W ThomasDavid Bar-Or
Feb 13, 2015·Critical Care : the Official Journal of the Critical Care Forum·Didier Payen, Houda Haloui
Jan 5, 2011·Critical Care Medicine·Julia Wendon
Nov 3, 2009·Proteomics·Marina GayJoaquin Abian
Apr 21, 2016·Biochemical and Biophysical Research Communications·Gregory W ThomasDavid Bar-Or
Jun 19, 2013·Analytical Biochemistry·David Bar-OrLeonard T Rael
Jul 8, 2016·Annals of Clinical and Translational Neurology·Faith H BrennanMarc J Ruitenberg
Sep 30, 2016·Orthopedics·John SchwappachKristin M Salottolo
Dec 22, 2015·Cell Transplantation·Yu-Hsun ChangDah-Ching Ding
Nov 22, 2017·International Journal of Environmental Research and Public Health·Chia-Jung HuangChih-Hsien Wang
Dec 9, 2017·Expert Review of Clinical Pharmacology·Ricard FerrerJosé Garnacho-Montero
Mar 14, 2013·Shock·Fernando Godinho ZampieriLuciano Cesar Pontes Azevedo
Nov 16, 2021·Journal of Internal Medicine·M A Wu, R Colombo

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