Commercial single-walled carbon nanotubes effects in fibrinolysis of human umbilical vein endothelial cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Yury Rodríguez-YáñezArnulfo Albores

Abstract

Recent studies have demonstrated that carbon nanotubes (CNTs) induce platelet aggregation, endothelial dysfunction and vascular thrombosis. However, there is little information on the effects of CNTs on fibrinolysis. We investigated the role of pristine-commercial single-walled carbon nanotubes (SWCNTs) with <3% Co content in fibrinolysis and their contribution to the induction of pro-thrombotic processes in human vein endothelial cells (HUVEC). SWCNTs alone produced concentration-dependent oxidation, as measured by a dithiothreitol oxidation assay. Internalized SWCNTs were located in HUVEC treated with 25 μg/ml using transmission electron microscopy, whereas treatment with 50 μg/ml compromised cell viability, and oxidative stress increased significantly at 5 μg/ml. The study showed that in HUVEC treated with 25 μg SWCNT/ml, fibrinolysis-related gene expression and protein levels had increased by 3-12 h after treatment (serpine-1: 13-fold; PLAT: 11-fold and PLAU: 2-fold), but only the PAI-1 protein was increased (1.5-fold), whereas tissue and urokinase plasminogen activator proteins (tPA and uPA, respectively) tended to decrease. In summary, pristine SWCNTs treatment resulted in evident HUVEC damage caused by cell fiber contact...Continue Reading

References

Apr 1, 1997·Current Opinion in Cell Biology·M KarinE Zandi
Aug 28, 1998·Journal of Inorganic Biochemistry·S LeonardX Shi
May 12, 2000·Hematology/oncology Clinics of North America·B Wiman
Jun 22, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·T Bachetti, L Morbidelli
Dec 10, 2000·The Journal of Biological Chemistry·M DingV Vallyathan
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·J P IrigoyenY Nagamine
Apr 16, 2002·Chemical Research in Toxicology·Yoshito KumagaiNobuhiro Shimojo
Sep 30, 2003·Journal of Toxicology and Environmental Health. Part a·Anna A ShvedovaPaul Baron
Dec 12, 2003·Journal of Toxicology and Environmental Health. Part a·Andrew D MaynardVincent Castranova
Jun 15, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kai HeckelAlwin E Goetz
Jan 15, 2005·Toxicology Letters·Nancy A Monteiro-RiviereJim E Riviere
Jun 14, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anna A ShvedovaPaul Baron
Sep 15, 2005·British Journal of Pharmacology·Anna RadomskiMarek W Radomski
May 16, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Furong TianHisatashi Kobayashi
Jun 15, 2006·Nano Letters·J M Wörle-KnirschH F Krug
Dec 16, 2006·Toxicology Letters·Peter WickArie Bruinink
Apr 5, 2007·Nature Protocols·Bruno BaudinMichel Vaubourdolle
Jun 9, 2007·Particle and Fibre Toxicology·Leonidas NtziachristosConstantinos Sioutas
Dec 1, 2007·Journal of Toxicology and Environmental Health. Part a·Elena R KisinAnna A Shvedova
Jun 24, 2008·Inhalation Toxicology·Jeong Hee HanIl Je Yu
Jul 26, 2008·Nature Nanotechnology·Kostas KostarelosAlberto Bianco
Jul 26, 2008·Nature Nanotechnology·Alexandra E PorterMark Welland
Jul 29, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·A A ShvedovaV E Kagan
Feb 28, 2009·Environmental Science & Technology·Edgar VidrioCort Anastasio
Mar 10, 2009·Toxicology and Applied Pharmacology·Valerie G WalkerPetia P Simeonova
Aug 25, 2009·Nanomedicine : Nanotechnology, Biology, and Medicine·Constantine P Firme, Prabhakar R Bandaru
Aug 25, 2009·Nanomedicine : Nanotechnology, Biology, and Medicine·Adriana AlbiniUgo Valbusa
Sep 24, 2009·Environmental and Molecular Mutagenesis·L M SargentS H Reynolds
Sep 29, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·David B Warheit
Feb 4, 2010·Inhalation Toxicology·Ji Hyun LeeIl Je Yu
May 1, 2010·Environmental Science & Technology·Ying WangSuzanne E Paulson

❮ Previous
Next ❯

Citations

May 5, 2016·Journal of Medicinal Chemistry·Reem AlshehriAdnan Memic
Nov 15, 2016·Chemical Society Reviews·Sandeep KumarKi-Hyun Kim
May 16, 2017·Oxidative Medicine and Cellular Longevity·Ying LiangYouchu Ma
Nov 21, 2018·Environmental Science and Pollution Research International·Hossein DinmohammadiTina Shahani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.