Abstract
Neutralizing antibodies (NAbs) form the basis of immunotherapeutic strategies against many important human viral infections. Accordingly, we studied the prevalence, titer, genotype-specificity, and mechanism of action of anti-polyomavirus BK (BKV) NAbs in commercially available human immune globulin (IG) preparations designed for intravenous (IV) use. Pseudovirions (PsV) of genotypes Ia, Ib2, Ic, II, III, and IV were generated by co-transfecting a reporter plasmid encoding luciferase and expression plasmids containing synthetic codon-modified VP1, VP2, and VP3 capsid protein genes into 293TT cells. NAbs were measured using luminometry. All IG preparations neutralized all BKV genotypes, with mean EC50 titers as high as 254 899 for genotype Ia and 6,666 for genotype IV. Neutralizing titers against genotypes II and III were higher than expected, adding to growing evidence that infections with these genotypes are more common than currently appreciated. Batch to batch variation in different lots of IG was within the limits of experimental error. Antibody mediated virus neutralizing was dose dependent, modestly enhanced by complement, genotype-specific, and achieved without effect on viral aggregation, capsid morphology, elution, or ...Continue Reading
References
Mar 9, 2004·Journal of Clinical Microbiology·Parmjeet RandhawaKaren Weck
Jun 23, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Melvin Berger
Feb 17, 2005·Transplantation·Noush Afarin FarasatiParmjeet Randhawa
Jul 22, 2005·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Gary L DavisUNKNOWN Collaborative Antiviral Study Group
Oct 11, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Sundaram HariharanBrian DuChateau
Jan 20, 2006·Transplantation·Alp SenerPatrick P W Luke
Apr 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H M WadeiM D Griffin
May 3, 2006·The Journal of Infectious Diseases·Francis BarinUNKNOWN Perinatal HIV Prevention Trial Group
Aug 19, 2007·Clinical Journal of the American Society of Nephrology : CJASN·Jodi M SmithRuth A McDonald
Aug 21, 2008·Journal of Medical Virology·C LuoP Randhawa
Aug 30, 2008·Clinical and Vaccine Immunology : CVI·P RandhawaR Viscidi
Sep 25, 2008·Transplant Infectious Disease : an Official Journal of the Transplantation Society·M KoukoulakiS Drakopoulos
Sep 30, 2008·Pediatric Transplantation·Ajay P SharmaJoanne Grimmer
Apr 9, 2009·Transplantation·Vikas R DharnidharkaKevin C Abbott
May 14, 2009·PLoS Pathogens·Ann J HessellDennis R Burton
May 19, 2009·Nature Medicine·Philip R JohnsonK Reed Clark
May 29, 2009·Journal of Clinical Microbiology·Sohrab BodaghiHans H Hirsch
Jul 9, 2009·Transplantation·Nina BabelPetra Reinke
Nov 13, 2009·Journal of Virology·Ann J HessellDennis R Burton
Jan 28, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J LevitskyUNKNOWN AST Infectious Diseases Community of Practice
May 22, 2010·Transplant Infectious Disease : an Official Journal of the Transplantation Society·U MaggioreC Buzio
Jun 24, 2010·Transplantation·Parmjeet S RandhawaYuchen Huang
Apr 13, 2011·PloS One·Jennifer D WatkinsRuth M Ruprecht
Nov 5, 2011·Journal of Medical Virology·Chunqing LuoParmjeet Randhawa
Nov 16, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J SellarésP F Halloran
Apr 19, 2012·PLoS Pathogens·Diana V PastranaChristopher B Buck
Apr 28, 2012·Clinical Proteomics·Rebecca KonietznyChris W Pugh
Jun 15, 2013·Journal of Medical Virology·J LedesmaP Muñoz
Jul 12, 2013·Journal of Virology·Diana V PastranaChristopher B Buck
Nov 6, 2013·Antiviral Research·Kevin J Whaley, Larry Zeitlin
Dec 24, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Corinna SchmittAlbert Heim
Jan 28, 2014·Archives of Disease in Childhood·Joanna MurrayMike Sharland
Citations
Feb 2, 2016·Néphrologie & thérapeutique·Antoine LanotBruno Hurault de Ligny
Jun 3, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H F WunderinkM C W Feltkamp
Jan 19, 2016·Nephrology·Jagadish S Jamboti
Oct 25, 2016·Seminars in Nephrology·Jennifer Trofe-Clark, Deirdre Sawinski
Oct 26, 2016·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Elizabeth SarmientoJavier Carbone
Oct 27, 2016·Viruses·Heidi BarthSamira Fafi-Kremer
Nov 2, 2016·Critical Reviews in Microbiology·Heidi BarthSamira Fafi-Kremer
Apr 14, 2017·Transplantation Direct·Kathy KableBrian John Nankivell
May 17, 2018·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Karel KrejciJosef Zadrazil
Mar 17, 2017·Clinical Microbiology Reviews·George R AmbalathingalRajiv Khanna
Dec 10, 2017·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Andrew D SanteusanioJudy Eun
Jun 5, 2019·Antimicrobial Agents and Chemotherapy·Aurélie VelaySamira Fafi-Kremer
Oct 17, 2019·Viruses·Fatima DakroubEtienne Brochot
Aug 3, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Ilies BenotmaneSophie Caillard
Mar 5, 2019·F1000Research·Abraham Cohen-BucayJean M Francis
May 10, 2017·Expert Review of Anti-infective Therapy·Jordi Bozzo, Juan I Jorquera
Apr 6, 2019·Open Forum Infectious Diseases·H F WunderinkM C W Feltkamp
Aug 22, 2020·International Journal of Nephrology and Renovascular Disease·Rajeev Sharma, Mareena Zachariah
Jan 12, 2020·Virology Journal·Elias Myrvoll LorentzenChristine Hanssen Rinaldo
Nov 5, 2015·Current Opinion in Organ Transplantation·Fasika M TedlaAmarpali Brar
Oct 28, 2019·Pediatric Transplantation·Kim Huynh Piburn, Samhar Al-Akash
Jun 20, 2020·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Ahmed SalehAhmed Halawa
Jan 13, 2021·Microorganisms·Carlo PerriconeRoberto Perricone
Oct 30, 2020·Therapeutics and Clinical Risk Management·Soichi MatsumuraRyoichi Imamura
Apr 23, 2020·Journal of Autoimmunity·Carlo PerriconeRoberto Gerli
Apr 4, 2021·Viruses·Chia-Lin ShenChih-Yu Yang
Oct 12, 2016·Microbiology Spectrum·Linda Cook
Apr 20, 2021·Transplant International : Official Journal of the European Society for Organ Transplantation·Bettina NaefThomas Schachtner
Apr 29, 2021·Transplantation·Thida Maung MyintGermaine Wong