PMID: 6974759Oct 1, 1981Paper

Committed T lymphocyte stem cells of rats. Characterization by surface W3/13 antigen and radiosensitivity

The Journal of Experimental Medicine
M J Dyer, S V Hunt

Abstract

The existence of stem cells committed to the T lymphoid lineage was deduced from studying how rat T and B stem cells differ in their expression of membrane W3/13 antigen and in their susceptibility in vivo to gamma irradiation. Stem cell activity of rat bone marrow and fetal liver was measured in long-term radiation chimeras using B and T cell alloantigenic surface markers to identify the progeny of donor cells. Monoclonal mouse anti-rat thymocyte antibody W3/13 labeled approximately 40% of fetal liver cells and 60-70% of young rat bone marrow cells (40% brightly, 25% dimly). Bright, dim, and negative cells were separated on a fluorescence-activated cell sorter. All B and T lymphoid stem cells in fetal liver were W3/13 bright, as were B lymphoid stem cells in bone marrow. W3/13 dim bone marrow had over half the T cell repopulating activity of unseparated marrow but gave virtually no B cell repopulation. In further experiments, the radiosensitivity of endogenous B and T lymphoid stem cells was determined by exposing host rats to between 4.5 and 10 Gy of gamma irradiation before repopulation with genetically marked marrow. The results depended on whether chimerism was assayed before day 50 or after day 100. At early times, a radi...Continue Reading

References

Nov 1, 1978·The Journal of Experimental Medicine·I GoldschneiderR J Morris
Apr 1, 1973·Cellular Immunology·J Sprent, A Basten
Apr 1, 1972·Journal of Cellular Physiology·H S MicklemD S Papworth
Aug 1, 1980·European Journal of Immunology·R J BrideauA F Williams
Jul 1, 1981·Cell and Tissue Kinetics·S V Hunt, M H Fowler

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Citations

Jan 1, 1990·Developmental and Comparative Immunology·Y FujikuraT Fukumoto
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D M HaigE E Jarrett
May 1, 1988·The Journal of Experimental Medicine·G J SpangrudeI L Weissman
Aug 3, 1999·The Journal of Experimental Medicine·M K WildP A van der Merwe
Jan 1, 1991·International Endodontic Journal·G BergenholtzM Jontell
Aug 1, 1986·Scandinavian Journal of Immunology·L M VaessenH J Schuurman
Nov 1, 1986·European Journal of Immunology·Y AiharaJ Klein

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