Common and metal-specific proteomic responses to cadmium and zinc in the metal tolerant ericoid mycorrhizal fungus Oidiodendron maius Zn

Metallomics : Integrated Biometal Science
Marco ChiapelloSilvia Perotto

Abstract

Although adaptive metal tolerance may arise in fungal populations in polluted soils, the mechanisms underlying metal-specific tolerance are poorly understood. Comparative proteomics is a powerful tool to identify variation in protein profiles caused by changing environmental conditions, and was used to investigate protein accumulation in a metal tolerant isolate of the ericoid mycorrhizal fungus Oidiodendron maius exposed to zinc and cadmium. Two-dimensional gel electrophoresis and shotgun proteomics followed by mass spectrometry lead to the identification of common and metal-specific proteins and pathways. Proteins selectively induced by cadmium exposure were molecular chaperons of the Hsp90 family, cytoskeletal proteins and components of the translation machinery. Zinc significantly up-regulated metabolic pathways related to energy production and carbohydrates metabolism, likely mirroring zinc adaptation of this fungal isolate. Common proteins induced by the two metal ions were the antioxidant enzyme Cu/Zn superoxide dismutase and ubiquitin. In mycelia exposed to zinc and cadmium, both proteomic techniques also identified agmatinase, an enzyme involved in polyamine biosynthesis. This novel finding suggests that, like plants, ...Continue Reading

References

Jan 1, 1989·Life Sciences·A J Khan, S C Minocha
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S KoyasuI Yahara
May 9, 2000·Nature·J M SharplesJ W Cairney
May 18, 2000·Biochemical and Biophysical Research Communications·K Igarashi, K Kashiwagi
Oct 3, 2001·The Journal of Biological Chemistry·J P WeiE B Gralla
Dec 13, 2001·Journal of Experimental Botany·J L Hall
May 9, 2002·Journal of Experimental Botany·Andres Schützendübel, Andrea Polle
Jan 16, 2003·Biochimica Et Biophysica Acta·Magdalena DudkowskaMałgorzata Manteuffel-Cymborowska
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Apr 9, 2005·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Pankaj S AroraGobinda Sarkar
Feb 1, 2006·FEMS Microbiology Letters·Marc BellionMichel Chalot
Jun 3, 2006·Annals of Botany·Sílvia FerreiraMaria Salomé Pais
Oct 10, 2006·Biotechnology Letters·Rubén AlcázarTeresa Altabella
Nov 4, 2006·Journal of Proteome Research·Bing ZhangNagiza F Samatova
Mar 16, 2007·Amino Acids·M D Groppa, M P Benavides
May 29, 2007·Nucleic Acids Research·Yuki MoriyaMinoru Kanehisa
Mar 1, 2008·Mycological Research·Andrés GárrizFernando L Pieckenstain
May 27, 2008·Biochemical and Biophysical Research Communications·Sandeep K SharmaPhilipp Christen
Jul 3, 2008·Planta·T KusanoY Takahashi
Aug 23, 2008·Journal of Molecular Biology·Ritwick SawarkarUtpal Tatu
Dec 17, 2008·Journal of Proteome Research·Pol KiefferJenny Renaut
Mar 12, 2010·Planta·Rubén AlcázarAntonio F Tiburcio
Apr 9, 2010·FEMS Microbiology Reviews·Robert Wysocki, Markus J Tamás
Jun 29, 2010·Journal of Proteome Research·Marco ChiapelloSilvia Perotto
Sep 16, 2010·FEMS Yeast Research·Laura Valdés-SantiagoJosé Ruiz-Herrera
Jan 19, 2011·Biotechnology Advances·Syed Sarfraz HussainKadambot H M Siddique
Apr 27, 2011·FEMS Microbiology Letters·Sean Doyle
Oct 7, 2011·Molecular Ecology·Nicolas Corradi, Christiane Charest

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Citations

Aug 30, 2019·Fungal Genetics and Biology : FG & B·Luis L Pérez-MozquedaErnestina Castro-Longoria

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