Common glycoproteins expressing polylactosamine-type glycans on matched patient primary and metastatic melanoma cells show different glycan profiles

Journal of Proteome Research
Mitsuhiro KinoshitaKazuaki Kakehi

Abstract

Recently, we reported comparative analysis of glycoproteins which express cancer-specific N-glycans on various cancer cells and identified 24 glycoproteins having polylactosamine (polyLacNAc)-type N-glycans that are abundantly present in malignant cells [ Mitsui et al., J. Pharm. Biomed. Anal. 2012 , 70 , 718 - 726 ]. In the present study, we applied the technique to comparative studies on common glycoproteins present in the matched patient primary and metastatic melanoma cell lines. Metastatic melanoma cells (WM266-4) contained a large amount of polyLacNAc-type N-glycans in comparison with primary melanoma cells (WM115). To identify the glycoproteins expressing these N-glycans, glycopeptides having polyLacNAc-type N-glycans were captured by a Datura stramonium agglutinin (DSA)-immobilized agarose column. The captured glycopeptides were analyzed by LC/MS after removing N-glycans, and some glycoproteins such as basigin, lysosome-associated membrane protein-1 (LAMP-1), and chondroitin sulfate proteoglycan 4 (CSPG4) were identified in both WM115 and WM266-4 cells. The expression level of polyLacNAc of CSPG4 in WM266-4 cells was significantly higher than that in WM115 cells. In addition, sulfation patterns of chondroitin sulfate (C...Continue Reading

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Citations

May 11, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elisabete FernandesLúcio Lara Santos
Jun 29, 2016·Molecular Aspects of Medicine·Poh-Choo PangGary F Clark
Nov 5, 2016·Expert Review of Gastroenterology & Hepatology·Srividya Venkitachalam, Kishore Guda
Mar 17, 2018·Glycobiology·Oliver M T Pearce
Dec 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kohya KudoKiyoko Fukami
Dec 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elisa TamburiniRoberto Perris
Jun 14, 2017·Cancer Cell·Praveen AgrawalEva Hernando
May 22, 2020·Journal of Proteome Research·Keita YamadaMitsuhiro Kinoshita

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